NLRX1 Protein (AA 87-975) (His tag)

Produktnummer ABIN3094177

Produktdetails

Target: NLRX1 (NLR Family Member X1 (NLRX1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 87-975

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses NLRX1 Protein ist gelabelt mit His tag.

Applikation: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: AEWFSRLPRE ERQFGPTFAL DTVHVDPVIR ESTPDELLRP PAELALEHQP PQAGLPPLAL SQLFNPDACG RRVQTVVLYG TVGTGKSTLV RKMVLDWCYG RLPAFELLIP FSCEDLSSLG PAPASLCQLV AQRYTPLKEV LPLMAAAGSH LLFVLHGLEH LNLDFRLAGT GLCSDPEEPQ EPAAIIVNLL RKYMLPQASI LVTTRPSAIG RIPSKYVGRY GEICGFSDTN LQKLYFQLRL NQPYCGYAVG GSGVSATPAQ RDHLVQMLSR NLEGHHQIAA ACFLPSYCWL VCATLHFLHA PTPAGQTLTS IYTSFLRLNF SGETLDSTDP SNLSLMAYAA RTMGKLAYEG VSSRKTYFSE EDVCGCLEAG IRTEEEFQLL HIFRRDALRF FLAPCVEPGR AGTFVFTVPA MQEYLAALYI VLGLRKTTLQ KVGKEVAELV GRVGEDVSLV LGIMAKLLPL RALPLLFNLI KVVPRVFGRM VGKSREAVAQ AMVLEMFREE DYYNDDVLDQ MGASILGVEG PRRHPDEPPE DEVFELFPMF MGGLLSAHNR AVLAQLGCPI KNLDALENAQ AIKKKLGKLG RQVLPPSELL DHLFFHYEFQ NQRFSAEVLS SLRQLNLAGV RMTPVKCTVV AAVLGSGRHA LDEVNLASCQ LDPAGLRTLL PVFLRARKLG LQLNSLGPEA CKDLRDLLLH DQCQITTLRL SNNPLTAAGV AVLMEGLAGN TSVTHLSLLH TGLGDEGLEL LAAQLDRNRQ LQELNVAYNG AGDTAALALA RAAREHPSLE LLHLYFNELS SEGRQVLRDL GGAAEGGARV VVSLTEGTAV SEYWSVILSE VQRNLNSWDR ARVQRHLELL LRDLEDSRGA TLNPWRKAQL LRVEGEVRAL LEQLGSSGS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NLRX1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NLRX1 (NLR Family Member X1 (NLRX1))

Andere Bezeichnung: NLRX1 (NLRX1 Produkte)

Synonyme: CLR11.3 Protein, DLNB26 Protein, NOD26 Protein, NOD5 Protein, NOD9 Protein, BC034204 Protein, RGD1311293 Protein, NLR family member X1 Protein, NLRX1 Protein, nlrx1 Protein, Nlrx1 Protein

Hintergrund: Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313). {ECO:0000269|PubMed:18200010, ECO:0000269|PubMed:18219313}.

Molekulargewicht: 98.7 kDa Including tag.

UniProt: Q86UT6

Pathways: SARS-CoV-2 Protein Interaktom