KIF24 Protein (AA 1-1368) (Strep Tag)

Produktnummer ABIN3093379

Produktdetails

Target: KIF24 (Kinesin Family Member 24 (KIF24))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1368

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses KIF24 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MASWLYECLC EAELAQYYSH FTALGLQKID ELAKITMKDY SKLGVHDMND RKRLFQLIKI IKIMQEEDKA VSIPERHLQT SSLRIKSQEL RSGPRRQLNF DSPADNKDRN ASNDGFEMCS LSDFSANEQK STYLKVLEHM LPDDSQYHTK TGILNATAGD SYVQTEISTS LFSPNYLSAI LGDCDIPIIQ RISHVSGYNY GIPHSCIRQN TSEKQNPWTE MEKIRVCVRK RPLGMREVRR GEINIITVED KETLLVHEKK EAVDLTQYIL QHVFYFDEVF GEACTNQDVY MKTTHPLIQH IFNGGNATCF AYGQTGAGKT YTMIGTHENP GLYALAAKDI FRQLEVSQPR KHLFVWISFY EIYCGQLYDL LNRRKRLFAR EDSKHMVQIV GLQELQVDSV ELLLEVILKG SKERSTGATG VNADSSRSHA VIQIQIKDSA KRTFGRISFI DLAGSERAAD ARDSDRQTKM EGAEINQSLL ALKECIRALD QEHTHTPFRQ SKLTQVLKDS FIGNAKTCMI ANISPSHVAT EHTLNTLRYA DRVKELKKGI KCCTSVTSRN RTSGNSSPKR IQSSPGALSE DKCSPKKVKL GFQQSLTVAA PGSTRGKVHP LTSHPPNIPF TSAPKVSGKR GGSRGSPSQE WVIHASPVKG TVRSGHVAKK KPEESAPLCS EKNRMGNKTV LGWESRASGP GEGLVRGKLS TKCKKVQTVQ PVQKQLVSRV ELSFGNAHHR AEYSQDSQRG TPARPASEAW TNIPPHQKER EEHLRFYHQQ FQQPPLLQQK LKYQPLKRSL RQYRPPEGQL TNETPPLFHS YSENHDGAQV EELDDSDFSE DSFSHISSQR ATKQRNTLEN SEDSFFLHQT WGQGPEKQVA ERQQSLFSSP RTGDKKDLTK SWVDSRDPIN HRRAALDHSC SPSKGPVDWS RENSTSSGPS PRDSLAEKPY CSQVDFIYRQ ERGGGSSFDL RKDASQSEVS GENEGNLPSP EEDGFTISLS HVAVPGSPDQ RDTVTTPLRE VSADGPIQVT STVKNGHAVP GEDPRGQLGT HAEYASGLMS PLTMSLLENP DNEGSPPSEQ LVQDGATHSL VAESTGGPVV SHTVPSGDQE AALPVSSATR HLWLSSSPPD NKPGGDLPAL SPSPIRQHPA DKLPSREADL GEACQSRETV LFSHEHMGSE QYDADAEETG LDGSWGFPGK PFTTIHMGVP HSGPTLTPRT GSSDVADQLW AQERKHPTRL GWQEFGLSTD PIKLPCNSEN VTWLKPRPIS RCLARPSSPL VPSCSPKTAG TLRQPTLEQA QQVVIRAHQE QLDEMAELGF KEETLMSQLA SNDFEDFVTQ LDEIMVLKSK CIQSLRSQLQ LYLTCHGPTA APEGTVPS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: KIF24 (Kinesin Family Member 24 (KIF24))

Andere Bezeichnung: KIF24 (KIF24 Produkte)

Synonyme: C9orf48 Protein, bA571F15.4 Protein, 4933425J19Rik Protein, 9430029L23Rik Protein, RGD1307723 Protein, kinesin family member 24 Protein, KIF24 Protein, Kif24 Protein

Hintergrund: Kinesin-like protein KIF24,FUNCTION: Microtubule-dependent motor protein that acts as a negative regulator of ciliogenesis by mediating recruitment of CCP110 to mother centriole in cycling cells, leading to restrict nucleation of cilia at centrioles. Mediates depolymerization of microtubules of centriolar origin, possibly to suppress aberrant cilia formation (PubMed:21620453). Following activation by NEK2 involved in disassembly of primary cilium during G2/M phase but does not disassemble fully formed ciliary axonemes. As cilium assembly and disassembly is proposed to coexist in a dynamic equilibrium may suppress nascent cilium assembly and, potentially, ciliar re-assembly in cells that have already disassembled their cilia ensuring the completion of cilium removal in the later stages of the cell cycle (PubMed:26290419). Plays an important role in recruiting MPHOSPH9, a negative regulator of cilia formation to the distal end of mother centriole (PubMed:30375385). {ECO:0000269|PubMed:21620453, ECO:0000269|PubMed:26290419, ECO:0000269|PubMed:30375385}.

Molekulargewicht: 151.9 kDa

UniProt: Q5T7B8