IB2 Protein (AA 1-824) (Strep Tag)

Produktnummer ABIN3093329

Produktdetails

Target: IB2 (JIP-2) (C-Jun-Amino-Terminal Kinase-Interacting Protein 2 (JIP-2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-824

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses IB2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MADRAEMFSL STFHSLSPPG CRPPQDISLE EFDDEDLSEI TDDCGLGLSY DSDHCEKDSL SLGRSEQPHP ICSFQDDFQE FEMIDDNEEE DDEDEEEEEE EEEGDGEGQE GGDPGSEAPA PGPLIPSPSV EEPHKHRPTT LRLTTLGAQD SLNNNGGFDL VRPASWQETA LCSPAPEALR ELPGPLPATD TGPGGAQSPV RPGCDCEGNR PAEPPAPGGT SPSSDPGIEA DLRSRSSGGR GGRRSSQELS SPGSDSEDAG GARLGRMISS ISETELELSS DGGSSSSGRS SHLTNSIEEA SSPASEPEPP REPPRRPAFL PVGPDDTNSE YESGSESEPD LSEDADSPWL LSNLVSRMIS EGSSPIRCPG QCLSPAPRPP GEPVSPAGGA AQDSQDPEAA AGPGGVELVD METLCAPPPP APAAPRPGPA QPGPCLFLSN PTRDTITPLW AAPGRAARPG RACSAACSEE EDEEDDEEEE DAEDSAGSPG GRGTGPSAPR DASLVYDAVK YTLVVDEHTQ LELVSLRRCA GLGHDSEEDS GGEASEEEAG AALLGGGQVS GDTSPDSPDL TFSKKFLNVF VNSTSRSSST ESFGLFSCLV NGEEREQTHR AVFRFIPRHP DELELDVDDP VLVEAEEDDF WFRGFNMRTG ERGVFPAFYA HAVPGPAKDL LGSKRSPCWV ERFDVQFLGS VEVPCHQGNG ILCAAMQKIA TARKLTVHLR PPASCDLEIS LRGVKLSLSG GGPEFQRCSH FFQMKNISFC GCHPRNSCYF GFITKHPLLS RFACHVFVSQ ESMRPVAQSV GRAFLEYYQE HLAYACPTED IYLE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: IB2 (JIP-2) (C-Jun-Amino-Terminal Kinase-Interacting Protein 2 (JIP-2))

Andere Bezeichnung: MAPK8IP2 (JIP-2 Produkte)

Synonyme: IB2 Protein, JIP2 Protein, PRKM8IPL Protein, 3230402N03Rik Protein, AI847694 Protein, AW049958 Protein, Jip2 Protein, R75148 Protein, mitogen-activated protein kinase 8 interacting protein 2 Protein, MAPK8IP2 Protein, Mapk8ip2 Protein

Hintergrund: C-Jun-amino-terminal kinase-interacting protein 2 (JIP-2) (JNK-interacting protein 2) (Islet-brain-2) (IB-2) (JNK MAP kinase scaffold protein 2) (Mitogen-activated protein kinase 8-interacting protein 2),FUNCTION: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity). {ECO:0000250}.

Molekulargewicht: 88.0 kDa

UniProt: Q13387