COL5A1 Protein (AA 38-1605) (His tag)

Produktnummer ABIN3091866

Produktdetails

Target: COL5A1 (Collagen, Type V, alpha 1 (COL5A1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 38-1605

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses COL5A1 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: QPADLLKVLD FHNLPDGITK TTGFCATRRS SKGPDVAYRV TKDAQLSAPT KQLYPASAFP EDFSILTTVK AKKGSQAFLV SIYNEQGIQQ IGLELGRSPV FLYEDHTGKP GPEDYPLFRG INLSDGKWHR IALSVHKKNV TLILDCKKKT TKFLDRSDHP MIDINGIIVF GTRILDEEVF EGDIQQLLFV SDHRAAYDYC EHYSPDCDTA VPDTPQSQDP NPDEYYTEGD GEGETYYYEY PYYEDPEDLG KEPTPSKKPV EAAKETTEVP EELTPTPTEA APMPETSEGA GKEEDVGIGD YDYVPSEDYY TPSPYDDLTY GEGEENPDQP TDPGAGAEIP TSTADTSNSS NPAPPPGEGA DDLEGEFTEE TIRNLDENYY DPYYDPTSSP SEIGPGMPAN QDTIYEGIGG PRGEKGQKGE PAIIEPGMLI EGPPGPEGPA GLPGPPGTMG PTGQVGDPGE RGPPGRPGLP GADGLPGPPG TMLMLPFRFG GGGDAGSKGP MVSAQESQAQ AILQQARLAL RGPAGPMGLT GRPGPVGPPG SGGLKGEPGD VGPQGPRGVQ GPPGPAGKPG RRGRAGSDGA RGMPGQTGPK GDRGFDGLAG LPGEKGHRGD PGPSGPPGPP GDDGERGDDG EVGPRGLPGE PGPRGLLGPK GPPGPPGPPG VTGMDGQPGP KGNVGPQGEP GPPGQQGNPG AQGLPGPQGA IGPPGEKGPL GKPGLPGMPG ADGPPGHPGK EGPPGEKGGQ GPPGPQGPIG YPGPRGVKGA DGIRGLKGTK GEKGEDGFPG FKGDMGIKGD RGEIGPPGPR GEDGPEGPKG RGGPNGDPGP LGPPGEKGKL GVPGLPGYPG RQGPKGSIGF PGFPGANGEK GGRGTPGKPG PRGQRGPTGP RGERGPRGIT GKPGPKGNSG GDGPAGPPGE RGPNGPQGPT GFPGPKGPPG PPGKDGLPGH PGQRGETGFQ GKTGPPGPPG VVGPQGPTGE TGPMGERGHP GPPGPPGEQG LPGLAGKEGT KGDPGPAGLP GKDGPPGLRG FPGDRGLPGP VGALGLKGNE GPPGPPGPAG SPGERGPAGA AGPIGIPGRP GPQGPPGPAG EKGAPGEKGP QGPAGRDGLQ GPVGLPGPAG PVGPPGEDGD KGEIGEPGQK GSKGDKGEQG PPGPTGPQGP IGQPGPSGAD GEPGPRGQQG LFGQKGDEGP RGFPGPPGPV GLQGLPGPPG EKGETGDVGQ MGPPGPPGPR GPSGAPGADG PQGPPGGIGN PGAVGEKGEP GEAGEPGLPG EGGPPGPKGE RGEKGESGPS GAAGPPGPKG PPGDDGPKGS PGPVGFPGDP GPPGEPGPAG QDGPPGDKGD DGEPGQTGSP GPTGEPGPSG PPGKRGPPGP AGPEGRQGEK GAKGEAGLEG PPGKTGPIGP QGAPGKPGPD GLRGIPGPVG EQGLPGSPGP DGPPGPMGPP GLPGLKGDSG PKGEKGHPGL IGLIGPPGEQ GEKGDRGLPG PQGSSGPKGE QGITGPSGPI GPPGPPGLPG PPGPKGAKGS SGPTGPKGEA GHPGPPGPPG PPGEVIQPLP IQASRTRRNI DASQLLDDGN GENYVDYA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human COL5A1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: COL5A1 (Collagen, Type V, alpha 1 (COL5A1))

Andere Bezeichnung: COL5A1 (COL5A1 Produkte)

Synonyme: cha1V Protein, COL5A1 Protein, AI413331 Protein, collagen type V alpha 1 chain Protein, collagen, type V, alpha 1 L homeolog Protein, collagen, type V, alpha 1 Protein, collagen alpha-1(V) chain Protein, COL5A1 Protein, col5a1.L Protein, col5a1 Protein, LOC100561308 Protein, Col5a1 Protein

Hintergrund: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.

Molekulargewicht: 154.4 kDa Including tag.

UniProt: P20908

Pathways: Growth Factor Binding