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ANXA2P2 Protein (AA 2-339) (His tag)

Crystallography grade ANXA2P2 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3089770
$9,343.16
Zzgl. Versandkosten $45.00 und $20.00 Trockeneis
Lieferung in 30 bis 35 Werktagen
  • Target
    ANXA2P2 (Annexin A2 Pseudogene 2 (ANXA2P2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-339
    Spezies
    Human
    Quelle
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses ANXA2P2 Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    STVHEILCKL SLEGDHSTPP SAYGSVKAYT NFDAERDALN IETAIKTKGV DEVTIVNIVT NRDNAQRQDI VFSYQRRTKK ELASALKSAL SGHLETVILG LLKTPAQYDA SELKASMKGL GTDEDSLIEI ICSRTNQELQ EINRVYKEMY KTDLEKDIIS DTSGDFRKLM VALAKGRRAE DGSVIDYELI DQDAQDLYDA GVKRKGTDVP KWISIMTERS VPHLQKVFDR YKSYSPYDML ESIRKEVKGD LENAFLNLVQ RIQNKPLYFA DQLYDSMKGK GTRDKVLIRI MVSRSEVDML KIRSEFKRKY GKSLYYYIQQ DTKGDYQKAL LYLCGGDD
    Produktmerkmale

    This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    N-terminal His-tag

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    50 mM Hepes, pH 7.8; 300 mM NaCl, 5 % glycerin
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ANXA2P2 (Annexin A2 Pseudogene 2 (ANXA2P2))
    Andere Bezeichnung
    ANXA2P2
    Synonyme
    ANX2L2 Protein, ANX2P2 Protein, LPC2B Protein, annexin A2 pseudogene 2 Protein, ANXA2P2 Protein
    Hintergrund
    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. {ECO:0000250}.
    Molekulargewicht
    39.5 kDa Including tag.
    UniProt
    A6NMY6
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