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Activin A Receptor, Type IB (ACVR1B) (AA 24-126) protein (His tag) Protein

Crystallography grade ACVR1B Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3088560
Zzgl. Versandkosten $45.00 und $20.00 Trockeneis
Lieferung in 50 Werktagen
  • Target Alle Activin A Receptor Type IB/ALK-4 (ACVR1B) Proteine anzeigen
    Activin A Receptor Type IB/ALK-4 (ACVR1B)
    AA 24-126
    • 11
    • 5
    • 2
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    Aufreinigungstag / Konjugat
    Dieses Activin A Receptor Type IB/ALK-4 Protein ist gelabelt mit His tag.
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ACVR1B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    0.22 μm filtered
    Protein is endotoxin free.
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Nur für Forschungszwecke einsetzbar
  • Format
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Avoid repeated freeze-thaw cycles.
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Unlimited (if stored properly)
  • Target
    Activin A Receptor Type IB/ALK-4 (ACVR1B)
    Andere Bezeichnung
    ACVR1B (ACVR1B Produkte)
    ACVR1B, alk4, skr2, alk-4, xalk4, actrib, acvrlk4, DKFZp469A1614, 6820432J04, ActR-IB, ActRIB, Acvrlk4, Alk4, SKR2, ACTRIB, ACVRLK4, ALK4, Skr2, axis, taram-a, tarama, wu:fb75f01, activin A receptor type 1B, Activin receptor type-1B, activin A receptor, type IB, activin A receptor, type 1B, activin A receptor type 1Ba, ACVR1B, acvr1b, acv1b, Acvr1b, acvr1ba
    Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2. {ECO:0000269|PubMed:12364468, ECO:0000269|PubMed:12639945, ECO:0000269|PubMed:18039968, ECO:0000269|PubMed:20226172, ECO:0000269|PubMed:8196624, ECO:0000269|PubMed:9032295, ECO:0000269|PubMed:9892009}.
    12.4 kDa Including tag.
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