ITIH3 Protein (AA 35-651) (His tag)
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- Target Alle ITIH3 Proteine anzeigen
- ITIH3 (Inter alpha Globulin Inhibitor H3 (ITIH3))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 35-651
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses ITIH3 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- Sequenz
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LPEGVANGIE VYSTKINSKV TSRFAHNVVT MRAVNRADTA KEVSFDVELP KTAFITNFTL TIDGVTYPGN VKEKEVAKKQ YEKAVSQGKT AGLVKASGRK LEKFTVSVNV AAGSKVTFEL TYEELLKRHK GKYEMYLKVQ PKQLVKHFEI EVDIFEPQGI SMLDAEASFI TNDLLGSALT KSFSGKKGHV SFKPSLDQQR SCPTCTDSLL NGDFTITYDV NRESPGNVQI VNGYFVHFFA PQGLPVVPKN VAFVIDISGS MAGRKLEQTK EALLRILEDM QEEDYLNFIL FSGDVSTWKE HLVQATPENL QEARTFVKSM EDKGMTNIND GLLRGISMLN KAREEHRIPE RSTSIVIMLT DGDANVGESR PEKIQENVRN AIGGKFPLYN LGFGNNLNYN FLENMALENH GFARRIYEDS DADLQLQGFY EEVANPLLTG VEMEYPENAI LDLTQNTYQH FYDGSEIVVA GRLVDEDMNS FKADVKGHGA TNDLTFTEEV DMKEMEKALQ ERDYIFGNYI ERLWAYLTIE QLLEKRKNAH GEEKENLTAR ALDLSLKYHF VTPLTSMVVT KPEDNEDERA IADKPGEDAE ATPVSPAMSY LTSYQPPQNP YYYVDGD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ITIH3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Güteklasse
- Crystallography grade
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- ITIH3 (Inter alpha Globulin Inhibitor H3 (ITIH3))
- Andere Bezeichnung
- ITIH3 (ITIH3 Produkte)
- Synonyme
- AW108094 Protein, Intin3 Protein, Itih-3 Protein, PAIHC3 Protein, H3P Protein, ITI-HC3 Protein, Itih3 Protein, inter-alpha trypsin inhibitor, heavy chain 3 Protein, inter-alpha-trypsin inhibitor heavy chain 3 Protein, Itih3 Protein, ITIH3 Protein
- Hintergrund
- May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
- Molekulargewicht
- 70.3 kDa Including tag.
- UniProt
- Q06033
- Pathways
- Glycosaminoglycan Metabolic Process
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