GRIK2 Protein (AA 657-819) (His tag)

Produktnummer ABIN3080974

Produktdetails

Target: GRIK2 (Glutamate Receptor, Ionotropic, Kainate 2 (GRIK2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 657-819

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses GRIK2 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: AFLTVERMES PIDSADDLAK QTKIEYGAVE DGATMTFFKK SKISTYDKMW AFMSSRRQSV LVKSNEEGIQ RVLTSDYAFL MESTTIEFVT QRNCNLTQIG GLIDSKGYGV GTPMGSPYRD KITIAILQLQ EEGKLHMMKE KWWRGNGCPE EESKEASALG VQN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human GRIK2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GRIK2 (Glutamate Receptor, Ionotropic, Kainate 2 (GRIK2))

Andere Bezeichnung: GRIK2 (GRIK2 Produkte)

Synonyme: GRIK5 Protein, eaa4 Protein, glr6 Protein, gluk6 Protein, glur6 Protein, grik2 Protein, mrt6 Protein, GluR6 Protein, grik2-A Protein, EAA4 Protein, GLR6 Protein, GLUK6 Protein, GLUR6 Protein, GluK2 Protein, MRT6 Protein, AW124492 Protein, Glur-6 Protein, Glur6 Protein, Glurbeta2 Protein, GRIK2 Protein, glutamate ionotropic receptor kainate type subunit 2 Protein, glutamate receptor, ionotropic, kainate 2 L homeolog Protein, glutamate receptor, ionotropic, kainate 2 Protein, glutamate receptor, ionotropic, kainate 2 (beta 2) Protein, GRIK2 Protein, grik2.L Protein, grik2 Protein, Grik2 Protein

Hintergrund: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2 (By similarity). {ECO:0000250}.

Molekulargewicht: 19.3 kDa Including tag.

UniProt: Q13002

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity