Desmoglein 1 Protein (DSG1) (AA 50-548) (His tag)

Produktnummer ABIN3079162

Produktdetails

Target: Desmoglein 1 (DSG1)

Protein-Typ: Recombinant

Proteineigenschaft: AA 50-548

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Desmoglein 1 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: MEWIKFAAAC REGEDNSKRN PIAKIHSDCA ANQQVTYRIS GVGIDQPPYG IFVINQKTGE INITSIVDRE VTPFFIIYCR ALNSMGQDLE RPLELRVRVL DINDNPPVFS MATFAGQIEE NSNANTLVMI LNATDADEPN NLNSKIAFKI IRQEPSDSPM FIINRNTGEI RTMNNFLDRE QYGQYALAVR GSDRDGGADG MSAECECNIK ILDVNDNIPY MEQSSYTIEI QENTLNSNLL EIRVIDLDEE FSANWMAVIF FISGNEGNWF EIEMNERTNV GILKVVKPLD YEAMQSLQLS IGVRNKAEFH HSIMSQYKLK ASAISVTVLN VIEGPVFRPG SKTYVVTGNM GSNDKVGDFV ATDLDTGRPS TTVRYVMGNN PADLLAVDSR TGKLTLKNKV TKEQYNMLGG KYQGTILSID DNLQRTCTGT ININIQSFGN DDRTNTEPNT KITTNTGRQE STSSTNYDTS TTSTDSSQVY SSEPGNGAKD LLSDNVHFGP HHHHHH

Spezifität: C-terminal His-tag

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human DSG1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount..

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

C-terminal His-tag

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 20 mM Hepes, pH 7.5; 150 mM NaCl; 2% Lauroylsarcosin; 1mM EDTA; 1mM DTT

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Desmoglein 1 (DSG1)

Andere Bezeichnung: DSG1 (DSG1 Produkte)

Synonyme: CDHF4 Protein, DG1 Protein, DSG Protein, PPKS1 Protein, SPPK1 Protein, DSG-1 Protein, DSG1 Protein, Dsg1b Protein, Dsg1c Protein, desmoglein 1 Protein, desmoglein 4 Protein, DSG1 Protein, DSG4 Protein, Dsg1 Protein

Hintergrund: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Molekulargewicht: 56.3 kDa Including tag.

UniProt: Q02413

Pathways: Cell-Cell Junction Organization