SMYD5 Protein (AA 1-418) (Strep Tag)

Produktnummer ABIN3077182

Produktdetails

Target: SMYD5 (SMYD Family Member 5 (SMYD5))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-418

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SMYD5 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MAASMCDVFS FCVGVAGRAR VSVEVRFVSS AKGKGLFATQ LIRKGETIFV ERPLVAAQFL WNALYRYRAC DHCLRALEKA EENAQRLTGK PGQVLPHPEL CTVRKDLHQN CPHCQVMYCS AECRLAATEQ YHQVLCPGPS QDDPLHPLNK LQEAWRSIHY PPETASIMLM ARMVATVKQA KDKDRWIRLF SQFCNKTANE EEEIVHKLLG DKFKGQLELL RRLFTEALYE EAVSQWFTPD GFRSLFALVG TNGQGIGTSS LSQWVHACDT LELKPQDREQ LDAFIDQLYK DIEAATGEFL NCEGSGLFVL QSCCNHSCVP NAETSFPENN FLLHVTALED IKPGEEICIS YLDCCQRERS RHSRHKILRE NYLFVCSCPK CLAEADEPNV TSEEEEEEEE EEEGEPEDAE LGDEMTDV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SMYD5 (SMYD Family Member 5 (SMYD5))

Andere Bezeichnung: SMYD5 (SMYD5 Produkte)

Synonyme: zgc:103480 Protein, SMYD5 Protein, smyd5 Protein, AW536703 Protein, NN8-4AG Protein, Rai15 Protein, Rrg1 Protein, RAI15 Protein, RRG1 Protein, ZMYND23 Protein, SMYD family member 5 Protein, SET and MYND domain-containing protein 5 Protein, SET and MYND domain containing 5 Protein, SMYD family member 5 L homeolog Protein, smyd5 Protein, SMYD5 Protein, Smyd5 Protein, LOC100380866 Protein, smyd5.L Protein

Hintergrund: Histone-lysine N-trimethyltransferase SMYD5 (EC 2.1.1.372) (Protein NN8-4AG) (Retinoic acid-induced protein 15) (SET and MYND domain-containing protein 5) ([histone H4]-lysine20 N-trimethyltransferase SMYD5),FUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4 to form trimethylated histone H4 lysine 20 (H4K20me3) which represents a specific tag for epigenetic transcriptional repression (By similarity). In association with the NCoR corepressor complex, is involved in the repression of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the formation of H4K20me3 at the gene promoters (By similarity). Plays an important role in embryonic stem (ES) cell self-renewal and differentiation (By similarity). Promotes ES cell maintenance by silencing differentiation genes through deposition of H4K20me3 marks (By similarity). Maintains genome stability of ES cells during differentiation through regulation of heterochromatin formation and repression of endogenous repetitive DNA elements by depositing H4K20me3 marks (PubMed:28951459). {ECO:0000250|UniProtKB:Q3TYX3, ECO:0000269|PubMed:28951459}.

Molekulargewicht: 47.3 kDa

UniProt: Q6GMV2