WDR26 Protein (AA 1-661) (Strep Tag)

Produktnummer ABIN3075401

Produktdetails

Target: WDR26 (WD Repeat Domain 26 (WDR26))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-661

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses WDR26 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequenz: MQANGAGGGG GGGGGGGGGG GGGGGQGQTP ELACLSAQNG ESSPSSSSSA GDLAHANGLL PSAPSAASNN SNSLNVNNGV PGGAAAASSA TVAAASATTA ASSSLATPEL GSSLKKKKRL SQSDEDVIRL IGQHLNGLGL NQTVDLLMQE SGCRLEHPSA TKFRNHVMEG DWDKAENDLN ELKPLVHSPH AIVVRGALEI SQTLLGIIVR MKFLLLQQKY LEYLEDGKVL EALQVLRCEL TPLKYNTERI HVLSGYLMCS HAEDLRAKAE WEGKGTASRS KLLDKLQTYL PPSVMLPPRR LQTLLRQAVE LQRDRCLYHN TKLDNNLDSV SLLIDHVCSR RQFPCYTQQI LTEHCNEVWF CKFSNDGTKL ATGSKDTTVI IWQVDPDTHL LKLLKTLEGH AYGVSYIAWS PDDNYLVACG PDDCSELWLW NVQTGELRTK MSQSHEDSLT SVAWNPDGKR FVTGGQRGQF YQCDLDGNLL DSWEGVRVQC LWCLSDGKTV LASDTHQRIR GYNFEDLTDR NIVQEDHPIM SFTISKNGRL ALLNVATQGV HLWDLQDRVL VRKYQGVTQG FYTIHSCFGG HNEDFIASGS EDHKVYIWHK RSELPIAELT GHTRTVNCVS WNPQIPSMMA SASDDGTVRI WGPAPFIDHQ NIEEECSSMD S
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: WDR26 (WD Repeat Domain 26 (WDR26))

Andere Bezeichnung: WDR26 (WDR26 Produkte)

Synonyme: si:ch211-153j24.6 Protein, wdr26 Protein, wu:fc89f06 Protein, wu:fk83a10 Protein, CDW2 Protein, GID7 Protein, MIP2 Protein, 1600024A01Rik Protein, AA693241 Protein, AI447817 Protein, AU044014 Protein, C77982 Protein, Gid7 Protein, RGD1565589 Protein, WD repeat domain 26 Protein, WD repeat domain 26b Protein, WD repeat domain 26 S homeolog Protein, WD repeat-containing protein 26 Protein, WDR26 Protein, wdr26b Protein, wdr26 Protein, wdr26.S Protein, LOC100182411 Protein, Wdr26 Protein

Hintergrund: WD repeat-containing protein 26 (CUL4- and DDB1-associated WDR protein 2) (Myocardial ischemic preconditioning up-regulated protein 2),FUNCTION: G-beta-like protein involved in cell signal transduction (PubMed:15378603, PubMed:19446606, PubMed:22065575, PubMed:23625927, PubMed:27098453, PubMed:26895380). Acts as a negative regulator in MAPK signaling pathway (PubMed:15378603). Functions as a scaffolding protein to promote G beta:gamma-mediated PLCB2 plasma membrane translocation and subsequent activation in leukocytes (PubMed:22065575, PubMed:23625927). Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972). Acts as a negative regulator of the canonical Wnt signaling pathway through preventing ubiquitination of beta-catenin CTNNB1 by the beta-catenin destruction complex, thus negatively regulating CTNNB1 degradation (PubMed:27098453). Serves as a scaffold to coordinate PI3K/AKT pathway-driven cell growth and migration (PubMed:26895380). Protects cells from oxidative stress-induced apoptosis via the down-regulation of AP-1 transcriptional activity as well as by inhibiting cytochrome c release from mitochondria (PubMed:19446606). Protects also cells by promoting hypoxia-mediated autophagy and mitophagy (By similarity). {ECO:0000250|UniProtKB:F1LTR1, ECO:0000269|PubMed:15378603, ECO:0000269|PubMed:19446606, ECO:0000269|PubMed:23625927, ECO:0000269|PubMed:26895380, ECO:0000269|PubMed:27098453, ECO:0000269|PubMed:29911972}.

Molekulargewicht: 72.1 kDa

UniProt: Q9H7D7