PLCE1 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2729133

Produktdetails

Target: PLCE1 (Phospholipase C, epsilon 1 (PLCE1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PLCE1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human PLCE1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: PLCE1 (Phospholipase C, epsilon 1 (PLCE1))

Andere Bezeichnung: Plce1 (PLCE1 Produkte)

Synonyme: PLCE1 Protein, NPHS3 Protein, PLCE Protein, PPLC Protein, 4933403A21Rik Protein, PLCepsilon Protein, Plce Protein, mKIAA1516 Protein, phospholipase C, epsilon 1 Protein, phospholipase C epsilon 1 Protein, PLCE1 Protein, plce1 Protein, Plce1 Protein

Hintergrund: This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]

Molekulargewicht: 258.5 kDa

NCBI Accession: NP_057425

Pathways: EGFR Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling