MBNL1 Protein (Transcript Variant 6) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2725584

Produktdetails

Target: MBNL1 (Muscleblind-like Protein 1 (MBNL1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Transcript Variant 6

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses MBNL1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human MBNL1 (transcript variant 6) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: MBNL1 Activity Verified in an mRNA Splicing Assay:,MBNL1 Activity Verified in an mRNA Splicing Assay:

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Referenzen

Echeverria, Cooper: "Muscleblind-like 1 activates insulin receptor exon 11 inclusion by enhancing U2AF65 binding and splicing of the upstream intron." in: Nucleic acids research, Vol. 42, Issue 3, pp. 1893-903, (2014) (PubMed).

Antigendetails

Target: MBNL1 (Muscleblind-like Protein 1 (MBNL1))

Andere Bezeichnung: Mbnl1 (MBNL1 Produkte)

Synonyme: MBNL1 Protein, zgc:153954 Protein, Mbnl Protein, mKIAA0428 Protein, EXP Protein, EXP35 Protein, EXP40 Protein, EXP42 Protein, MBNL Protein, muscleblind like splicing regulator 1 Protein, muscleblind-like splicing regulator 1 Protein, muscleblind like splicing factor 1 Protein, MBNL1 Protein, mbnl1 Protein, Mbnl1 Protein

Hintergrund: This gene encodes a member of the muscleblind protein family which was initially described in Drosophila melanogaster. The encoded protein is a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Mice lacking this gene exhibited muscle abnormalities and cataracts. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. The different isoforms are thought to have different binding specificities and/or splicing activities.

Molekulargewicht: 36.8 kDa

NCBI Accession: NP_997179

Pathways: Ribonucleoprotein Complex Subunit Organization