Isocitrate Dehydrogenase Protein (IDH) (His tag)

Produktnummer ABIN2723845

Produktdetails

Target: Isocitrate Dehydrogenase (IDH)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Insect cells (Sf9)

Aufreinigungstag / Konjugat: Dieses Isocitrate Dehydrogenase Protein ist gelabelt mit His tag.

Applikation: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Isocitrate dehydrogenase / IDH (C-term polyhistidine tag) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: Enzymatic activities were determined by monitoring NADPH formation based on the absorbance at 345nm. The reaction was carried out at 37? for 10 minutes in the presence of isocitrate as a substrate and NADP as a cofactor. The data which presented a good linear relation on the curve was used to calculate the specific activity, and one unit is defined as converting 1.0 umole of NADP to NADPH per min at 37?. In summary, the wildtype IDH1 produced from HEK293 cells and insect cells are active while the R132H mutant or the WT/R132H heterodimers are inactive.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. < td valign=top>

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: Isocitrate Dehydrogenase (IDH)

Andere Bezeichnung: Isocitrate Dehydrogenase,idh (IDH Produkte)

Synonyme: CG7176 Protein, CT22171 Protein, Dmel\\CG7176 Protein, IDH Protein, IDH-NADP Protein, Idh-NADP Protein, idh Protein, isocitrate dehydrogenase Protein, l(3)L3852 Protein, F20D21.16 Protein, F20D21_16 Protein, SMU.672 Protein, icd Protein, Isocitrate dehydrogenase Protein, isocitrate dehydrogenase Protein, isocitrate dehydrogenase, NADP-dependent Protein, Monomeric isocitrate dehydrogenase Protein, ankyrin repeat domain 26-like Protein, Idh Protein, ICDH Protein, idh Protein, icd Protein, SSO_RS10660 Protein, JK_RS08640 Protein, LOC618625 Protein

Hintergrund: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Molekulargewicht: 47 kDa

NCBI Accession: NP_005887