PDX1 Protein (His tag)

Produktnummer ABIN2723515

Produktdetails

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PDX1 Protein ist gelabelt mit His tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human Insulin promoter factor 1 (N-term HIS tag) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the N-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Andere Bezeichnung: Insulin Promoter Factor 1 (PDX1 Produkte)

Synonyme: GSF Protein, IDX-1 Protein, IPF1 Protein, IUF1 Protein, MODY4 Protein, PDX-1 Protein, STF-1 Protein, ipf1 Protein, PDX1 Protein, IPF-1 Protein, Ipf1 Protein, Mody4 Protein, pdx-1 Protein, Idx1 Protein, Stf1 Protein, zgc:100874 Protein, XlHbox-8 Protein, xlhbox8 Protein, pancreatic and duodenal homeobox 1 Protein, pancreatic and duodenal homeobox 1 S homeolog Protein, PDX1 Protein, pdx1 Protein, Pdx1 Protein, pdx1.S Protein

Hintergrund: Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. [UniProtKB/Swiss-Prot Function]

Molekulargewicht: 30.6 kDa

NCBI Accession: NP_000200

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location