GDF15 Protein

Produktnummer ABIN2721778

Produktdetails

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: CHO Cells

Applikation: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human GDF15 protein expressed in Cell Culture.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 osetoblast cells. The expected ED50 for this effect is 1.0-3.0 μg/ml

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 10 mM Sodium Citrate, pH 3.0

Handhabung: Centrifuge the vial prior to opening. Reconstitute the protein in water to a concnetration of 0.1 ~ 1.0 mg/mL. Do not vertex. For extended storage, it is recommended to further dilute in a proper buffer containing a carrier protein such as 0.1 % BSA, and store in working aliquots at -20 °C or -80 °C

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Andere Bezeichnung: Gdf15 (GDF15 Produkte)

Synonyme: GDF15 Protein, GDF-15 Protein, MIC-1 Protein, MIC1 Protein, NAG-1 Protein, PDF Protein, PLAB Protein, PTGFB Protein, SBF Protein, growth differentiation factor 15 Protein, GDF15 Protein, Gdf15 Protein

Hintergrund: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress.

Molekulargewicht: 12 kDa

NCBI Accession: NP_004855

Pathways: SARS-CoV-2 Protein Interaktom