CBP Protein (AA 1081-1197) (His tag,DYKDDDDK Tag)

Produktnummer ABIN2669638

Produktdetails

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1081-1197

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses CBP Protein ist gelabelt mit His tag,DYKDDDDK Tag.

Applikation: Binding Studies (Bind), Screening Assay (ScA)

Produktmerkmale: The peptide corresponding to amino acids 1081 - 1197 that contains the bromodomain sequences of CREBBP (accession number NM_004380.2) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 18.7 kDa. It shows binding specificity for acetylated H2BK85, H3K9/K14, H3K14, H3K36, H3K56, H3S10/K14/K18, H4K12, H4K20 and H4K44, as well as acetylated p53-K382 , MyoD-K99 and MyoD-K99/K102.

Anwendungsinformationen

Applikationshinweise: Recombinant CREBBP (1081-1197) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Andere Bezeichnung: CREBBP (CREBBP Produkte)

Synonyme: CBP Protein, KAT3A Protein, RSTS Protein, AW558298 Protein, CBP/p300 Protein, p300/CBP Protein, RTS Protein, cbp Protein, crebbp Protein, kat3a Protein, rsts Protein, hmm291030 Protein, Nejire Protein, CREB binding protein Protein, CREB binding protein L homeolog Protein, histone acetyltransferase p300 Protein, CREBBP Protein, Crebbp Protein, crebbp.L Protein, LOC100123220 Protein

Hintergrund: CREB-binding protein (CREBBP) or CBP, is a protein known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. CREBBP shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. The CREBBP bromodomain recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. CREBBP has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. CREBBP acetylates histones as well as non-histone proteins, like NCOA3 coactivator. In addition, CREBBP binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. CREBBP also has been shown to act as a coactivator of ALX1 in the presence of p300.

Molekulargewicht: 18.7 kDa

Pathways: T-Zell Rezeptor Signalweg, Interferon-gamma Pathway, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha