KAT2B Protein (AA 715-829) (His tag,DYKDDDDK Tag)

Produktnummer ABIN2669635

Produktdetails

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 715-829

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses KAT2B Protein ist gelabelt mit His tag,DYKDDDDK Tag.

Applikation: Binding Studies (Bind), Screening Assay (ScA)

Produktmerkmale: The peptide corresponding to amino acids 715 - 829 that contains the bromodomain sequences of PCAF (accession number NM_003884.4) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 19.8 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K36, H4K8, H4K16, H4K20 and TATK50.

Anwendungsinformationen

Applikationshinweise: Recombinant PCAF (715-829) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Andere Bezeichnung: PCAF (KAT2B Produkte)

Synonyme: PCAF Protein, pcaf Protein, si:ch211-1j13.2 Protein, zgc:161980 Protein, gb:dq017634 Protein, im:7156024 Protein, CAF Protein, P Protein, P/CAF Protein, A930006P13Rik Protein, AI461839 Protein, AW536563 Protein, Pcaf Protein, p/CAF Protein, lysine acetyltransferase 2B Protein, K(lysine) acetyltransferase 2B Protein, K(lysine) acetyltransferase 2A Protein, KAT2B Protein, kat2b Protein, kat2a Protein, Kat2b Protein

Hintergrund: P300/CBP-associated factor (PCAF), also known as K (lysine) acetyltransferase 2B (KAT2B), is a transcriptional coactivator associated with p53 that contains acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. PCAF competes with E1A for binding to p300/CBP, which associate with various sequence-specific factors involved in cell growth and/or differentiation, including c-Jun and the adenoviral oncoprotein E1A. PCAF exerts histone acetyltransferase activity in association with core histones (H3 and H4) and with nucleosome core particles, and functions to promote transcriptional activation. PCAF has also been shown to inhibit cell cycle progression and to counteract the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, PCAF is recruited by the viral protein TAT to regulate TAT's transactivating activity and may help induce chromatin remodeling of proviral genes.

Molekulargewicht: 19.8 kDa

Pathways: p53 Signalweg, Regulation of Carbohydrate Metabolic Process