TRIM33 Protein (AA 959-1059) (His tag,DYKDDDDK Tag)

Produktnummer ABIN2669632

Dieses Recombinant TRIM33-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für TRIM33 Protein (AA 959-1059) (His tag,DYKDDDDK Tag) (ABIN2669632)

Target: TRIM33 (Tripartite Motif Containing 33 (TRIM33))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Binding Studies (Bind), Screening Assay (ScA)

Produktdetails

Proteineigenschaft: AA 959-1059

Aufreinigungstag / Konjugat: Dieses TRIM33 Protein ist gelabelt mit His tag,DYKDDDDK Tag.

Produktmerkmale: The peptide corresponding to amino acids 959 - 1059 that contains the bromodomain sequences of TRIM33 (accession number NP_056990.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 17.7 kDa.

Anwendungsinformationen

Applikationshinweise: Recombinant TRIM33 (959-1069) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: TRIM33 (Tripartite Motif Containing 33 (TRIM33))

Andere Bezeichnung: TRIM33

Hintergrund: Tripartite motif-containing 33 (TRIM33) protein, also known as TIF1γ, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1α), TRIM28 (TIF1β) and TRIM33 (TIF1γ) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM33 is an E3 ubiquitin protein ligase that promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. TRIM33 does not affect SMAD4 levels but rather acts as an inhibitor of the SMAD4-dependent TGFβ/BMP signaling cascade by monoubiquitinating SMAD4 and hampering its ability to form a stable complex with activated SMAD2/3. TRIM33 may also act as a transcriptional corepressor. It has also been shown to associate with SMAD2 and SMAD3 and stimulate differentiation of hematopoietic stem cells. Additionally, TRIM33 associates with TRIM24 and plays a role in the control of cell proliferation.

Molekulargewicht: 17.7 kDa