HIV-1 Tat Protein

Produktnummer ABIN2669349

Produktdetails

Target: HIV-1 Tat

Protein-Typ: Recombinant

Spezies: Human Immunodeficiency Virus (HIV)

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), SDS-PAGE (SDS)

Reinheit: > 90 %

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: The protein should be reconstituted in apirogenic sterile water or 1X PBS.

Buffer: 0.1 % glycerol

Lagerung: 4 °C

Antigendetails

Target: HIV-1 Tat

Andere Bezeichnung: HIV1 Tat (HIV-1 Tat Produkte)

Substanzklasse: Viral Protein

Hintergrund: The redox state of cysteine-rich region of HIV tat protein is known to play a crucial role in tat biological activity. The cysteine-rich region, encompassing seven cysteine residues, is highly conserved among the different HIV-1 strains and isolates. Its redox state is known to be important for protein function and activity. In particular, variation of the tat cysteine motif known as "cysteine 30-cysteine 31" (C30C31) is associated with clade-specific tat neurotoxicity. Formation of tat homodimers also depends on the oxidation state of the protein, as dimerization requires metal ions binding to free sulphydryl groups present in the cysteine region. In the presence of oxygen and in absence of reducing agents, tat protein rapidly oxidises forming intra-molecular disulfide bonds which prevent the interaction with metal ions. Further, oxidation induces the formation of inter-molecular disulfide bonds causing tat multimerization and aggregation. Due to these effects, oxidation hampers or abrogates tat biological activity.