Siglec E Protein (AA 20-355, C-Term) (Fc Tag)

Produktnummer ABIN2666859

Produktdetails

Target: Siglec E (Siglece) (Sialic Acid Binding Ig-Like Lectin E (Siglece))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 20-355, C-Term

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Siglec E Protein ist gelabelt mit Fc Tag.

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 1.0 EU per μg of protein as determine by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Recombinant mouse Siglec E is able to agglutinate human red blood cells with an ED50 of 0.625 - 2.5 μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Konzentration: 100 μg/mL

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: Siglec E (Siglece) (Sialic Acid Binding Ig-Like Lectin E (Siglece))

Andere Bezeichnung: Siglec E (Siglece Produkte)

Synonyme: Cd170 Protein, Siglec12 Protein, Siglec5 Protein, Siglec9 Protein, Siglecl1 Protein, mSiglec-E Protein, sialic acid binding Ig-like lectin E Protein, Siglece Protein

Hintergrund: Siglecs (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region containing a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. The cytoplasmic tails of all siglecs have one or more tyrosines within potential signalling motifs. Siglecs are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Studies suggest that siglecs may participate in cell-cell interactions or act as receptors for the entry of viral or bacterial pathogens. In addition, the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domain indicates that these molecules may play a role in the suppression of immunoreceptor signaling. The siglecs can be classified into two subgroups, with Siglec-1, -2, and -4 as one group and a Siglec-3/CD33-related subgroup (Siglec-3, and -5 through -13) as the second. Siglec E is a mouse CD33-related siglec that selectively regulates early recruitment of neutrophils to the lung in acute lung inflammation induced by lipopolysaccharide. Siglec E-deficient mice exhibit exaggerated neutrophil recruitment that is reversible by using a blockade of the β2 integrin, CD11b. In addition, sialidase treatment of fibrinogen reverses the suppressive effect of Siglec E on CD11b signaling, suggesting that sialic acid recognition by Siglec E is required for its inhibitory function. These findings indicate that Siglec E is an important negative regulator of neutrophil recruitment to the lung and β2 integrin-dependent signaling.

Molekulargewicht: This 570 amino acid recombinant protein has a predicted molecular mass of approximately 63 kDa. The protein migrates at about 80 - 90 kDa by SDS-PAGE in DTT-reducing conditions and about 180 - 200 kDa in non-reducing conditions. The predicted N-terminal a