HIV-1 p24 Protein

Produktnummer ABIN2452194

Produktdetails

Target: HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))

Protein-Typ: Recombinant

Spezies: Human Immunodeficiency Virus (HIV)

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Dot Blot (DB), Functional Studies (Func)

Reinheit: > 90 % purity by SDS-PAGE (CBB staining)

Anwendungsinformationen

Applikationshinweise: 1) SDS-PAGE as a p24 marker
2) In ELISA assay as a standard in titration of p24 antigens
3) As a standard for p24 in Western blotting.
4) It can be used in the studies of structure and function of HIV-1 virus as it constitutes HIV-1 core as a capsid protein since it is soluble under physiological conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 50 % glycerol, 20 mM Tris-HCl ( pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol

Lagerung: -20 °C/-80 °C

Referenzen

Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).

Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).

Tanaka, Saitoh, Nakata, Shinagawa: "A simple method for overproduction and purification of p24 Gag protein of human immunodeficiency virus type 1." in: Microbiology and immunology, Vol. 36, Issue 8, pp. 823-31, (1993) (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).

Antigendetails

Target: HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))

Andere Bezeichnung: HIV-1 Gag p24 (HIV-1 p24 Produkte)

Substanzklasse: Viral Protein

Hintergrund: HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus, HIV-1, and is produced by digestion of its precursor, Gag p55, by HIV-1 protease. This protein is indispensable for reproduction of AIDS virus and constitutes an essential element in the virus particle. As this protein is detectable from the early stage of AIDS virus infection, and reflects the amount of virus in the blood, it is used as a marker for observing the patient's condition during and after treatment. This protein was over-expressed as a soluble recombinant protein in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus, subtype B, and highly purified by several steps of chromatography. Its molecular weight is 24 kD, same as that of p24 purified from HIV-1 virus particles.