SEMA4D/CD100 Protein (AA 22-734) (Fc Tag)

Produktnummer ABIN2181739

Produktdetails

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Protein-Typ: Recombinant

Proteineigenschaft: AA 22-734

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SEMA4D/CD100 Protein ist gelabelt mit Fc Tag.

Sequenz: AA 22-734

Produktmerkmale: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 105.4 kDa. The protein migrates as 120-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >95 % as determined by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, pH 7.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Referenzen

Zou, Jiang, Dissanayaka, Heng, Xu, Gong, Huang, Zhang: "Sema4D/PlexinB1 promotes endothelial differentiation of dental pulp stem cells via activation of AKT and ERK1/2 signaling." in: Journal of cellular biochemistry, Vol. 120, Issue 8, pp. 13614-13624, (2019) (PubMed).

Zou, Dissanayaka, Jiang, Wang, Heng, Huang, Zhang: "Semaphorin 4D Enhances Angiogenic Potential and Suppresses Osteo-/Odontogenic Differentiation of Human Dental Pulp Stem Cells." in: Journal of endodontics, Vol. 43, Issue 2, pp. 297-305, (2018) (PubMed).

Antigendetails

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Andere Bezeichnung: Semaphorin 4D (SEMA4D Produkte)

Synonyme: C9orf164 Protein, CD100 Protein, M-sema-G Protein, SEMAJ Protein, coll-4 Protein, Semacl2 Protein, Semaj Protein, Semcl2 Protein, COLL-4 Protein, semaphorin 4D Protein, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D Protein, Sema4d Protein, SEMA4D Protein

Hintergrund: Cluster of Differentiation 100 (CD100) is also known as Semaphorin-4D (SEMA4D), is a single-pass type I membrane protein which belongs to the semaphorin family, and is a human 150- kDa homodimer expressed at the surface of most hemopoietic cells. Semaphorin genes encode soluble and membrane-bound proteins, most of which have been shown to act as chemorepellents on growth cone guidance. CD100 is discrete, as it is a transmembrane leukocyte surface molecule that can also exist in a soluble form. Semaphorin 4D (Sema 4D) is an axon guidance molecule which is secreted by oligodendrocytes and induces growth cone collapse in the central nervous system. By binding plexin B1 receptor it functions as an R-Ras GTPase-activating protein (GAP) and repels axon growth cones in both the mature central nervous system. In the immune system, CD100 binds CD72 to activate B cells and dendritic cells, though much about this interaction is still under investigation. It is involved in oligodendrogenesis during development and during recovery from ischemic injury.

Molekulargewicht: 105.4 kDa

Pathways: Regulation of Cell Size