Recombinant Human Early Placenta Insulin-Like Peptide/INSL4 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ala26-Thr139) of Human INSL4 fused with a 6His tag at the C-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
Crystallography grade
INSL4
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS, Crys
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM Tris,150 mM NACl, pH 8.0.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
EPIL Protein, PLACENTIN Protein, insulin like 4 Protein, INSL4 Protein
Sub Type
Fusionprotein
Hintergrund
Early Placenta Insulin-Like Peptide (INSL4) belongs to the insulin family. INSL4 is expressed in the early placental cytotrophoblast and syncytiotrophoblast INSL4 is a secreted protein and a precursor that undergoes post-translational cleavage to produce 3 polypeptide chains, A-C, that form tertiary structures composed of either all three chains, or just the A and B chains. INSL4 plays an important role in the development of trophoblast and in the regulation of bone formation. Alternative Names: Early Placenta Insulin-Like Peptide, EPIL, Insulin-Like Peptide 4, Placentin, INSL4