TNF alpha Protein

Produktnummer ABIN1305133

Recombinant TNF alpha-Protein exprimiert in Escherichia coli (E. coli) und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für TNF alpha Protein (ABIN1305133)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Applikation: ELISA, Functional Studies (Func)

Reinheit: ≥ 95 %

Produktdetails

Marke: BD Pharmingen™

Spezifität: Bioassay: Activity Range Tested by MTT/Actinomycin D Cytolysis Assay using L929 indicator cells
Specific Activity: 0.25 - 5.0 x 10^9 Units /mg
ED50: 2 - 40 pg/ml
Observed Dose response: 250 fold
The Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, titrate the reagent to obtain optimal results.

Produktmerkmale: Recombinant rat TNF is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 2 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant rat TNF was purified by immunoaffinity chromatography and yielded a TNF protein fraction which was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Aufreinigung: Immunoaffinity Chromatography
Recombinant rat TNF is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing bovine serum albumin, with no preservatives. Recombinant rat TNF is ≥ 95 % pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: The endotoxin level is ≤ 0.1 ng/µg of TNF protein, as measured in a chromogenic LAL assay.

Anwendungsinformationen

Applikationshinweise: ELISA Standard: Recombinant rat TNF is useful as a quantitative standard for measuring rat TNF protein levels in a TNF specific sandwich ELISA with purified TN3-19.12 antibody (Cat. No. 557516) as a capture antibody and biotinylated rabbit anti-mouse/rat TNF antibody (Cat. No. 557432) as the detection antibody. To obtain linear standard curves, doubling dilutions of rat TNF standard from ~10 ng - 0.1 ng/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. For measuring rat TNF in serum or plasma the rat TNF OptEIA™ Set (Cat. No. 558535) and rat TNF OptEIA™ Kit (Cat. No. 560479) are specially formulated and recommended. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

Kommentare:

BD Pharmingen™ Recombinant Rat TNF - Reactivity Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Konservierungsmittel: Azide free

Handhabung: Tumor Necrosis Factor (TNF, formerly known as TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. Rat TNF is a 17.4 kD protein containing 157 amino acid residues.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen

Jäättelä: "Biologic activities and mechanisms of action of tumor necrosis factor-alpha/cachectin." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 64, Issue 6, pp. 724-42, (1991) (PubMed).

Hogan, Vogel: "Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 12, pp. 4196-202, (1989) (PubMed).

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF

Hintergrund: Tumor Necrosis Factor (TNF, formerly known as TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. Rat TNF is a 17.4 kD protein containing 157 amino acid residues.

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome