BUB3 Protein (His tag)
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- Target Alle BUB3 Proteine anzeigen
- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses BUB3 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Reinheit
- > 85 % by SDS - PAGE
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Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-328) protein (GST tag)
BUB3 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AA, AP
Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-328) protein (Strep Tag)Crystallography grade BUB3 Spezies: Human Wirt: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-326) protein (Strep Tag)Crystallography grade BUB3 Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)BUB3 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Kommentare
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Synonyms: Mitotic checkpoint protein BUB3, BUB3L, hBUB3
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M Urea, 10% glycerol
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Avoid repeated freezing and thawing cycles.
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- Target
- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
- Andere Bezeichnung
- BUB3 (BUB3 Produkte)
- Synonyme
- BUB3L Protein, hBUB3 Protein, Aa2-050 Protein, xbub3 Protein, AU019800 Protein, AU021329 Protein, AU043350 Protein, AW146323 Protein, C78067 Protein, BUB3, mitotic checkpoint protein Protein, BUB3 mitotic checkpoint protein Protein, BUB3 mitotic checkpoint protein L homeolog Protein, BUB3 Protein, Bub3 Protein, bub3.L Protein
- Hintergrund
- Mitotic checkpoint protein BUB3, also known as BUB3, is a conserved component of the mitotic spindle assembly complex (MCC). The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. Recombinant human BUB3 protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molekulargewicht
- 39.5 kDa (351aa)
- NCBI Accession
- NP_004716
- Pathways
- Maintenance of Protein Location
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