POLR2E Protein (His tag)

Produktnummer ABIN1098477

Produktdetails

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses POLR2E Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, RPABC1, RPB5, XAP4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol, 1 MM DTT

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Andere Bezeichnung: POLR2E (POLR2E Produkte)

Synonyme: MGC68604 Protein, polr2e Protein, wu:fk97g10 Protein, zgc:101098 Protein, 2410021N14Rik Protein, 25kDa Protein, AW208866 Protein, RPB5 Protein, XAP4 Protein, RPABC1 Protein, hRPB25 Protein, hsRPB5 Protein, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa S homeolog Protein, RNA polymerase II subunit E Protein, polymerase (RNA) II (DNA directed) polypeptide E, b Protein, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa Protein, polymerase (RNA) II (DNA directed) polypeptide E Protein, DNA-directed RNA polymerases I, II, and III subunit RPABC1 Protein, polr2e.S Protein, POLR2E Protein, polr2eb Protein, polr2e Protein, Polr2e Protein, rpb-5 Protein

Hintergrund: POLR2E, also as known as DNA-directed RNA polymerases I, II, and III subunit RPABC1, belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. POLR2E is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 27.1kDa (233aa), confirmed by MALDI-TOF

NCBI Accession: NP_002686

Pathways: Regulatorische RNA Pathways