POLR2J Protein (His tag)

Produktnummer ABIN7279994

Produktdetails

Target: POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses POLR2J Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: DNA-directed RNA polymerase II subunit RPB11-a, hRPB14, POLR2J1, RPB11, RPB11A, RPB11m

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol, 5 MM DTT, and 2 mM EDTA

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))

Andere Bezeichnung: POLR2J (POLR2J Produkte)

Synonyme: POLR2J1 Protein, RPB11 Protein, RPB11A Protein, RPB11m Protein, hRPB14 Protein, POLR2J2 Protein, 14.5kDa Protein, Polr2i Protein, Rpb11a Protein, Rpo2-4 Protein, RNA polymerase II subunit J Protein, polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa S homeolog Protein, polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa Protein, polymerase (RNA) II (DNA directed) polypeptide J Protein, POLR2J Protein, Polr2j Protein, polr2j.S Protein, polr2j Protein

Hintergrund: POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit, together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. Recombinant human POLR2J protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 15.7 kDa (140aa) confirmed by MALDI-TOF

NCBI Accession: NP_006225

Pathways: Regulatorische RNA Pathways