MBD3 Protein (His tag)

Produktnummer ABIN1098411

Produktdetails

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses MBD3 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: Methyl-CpG-binding domain protein 3, Methyl CpG binding domain protein 3, Methyl-CpG-binding protein MBD3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol,1 MM DTT, 1 MM EDTA

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Andere Bezeichnung: MBD3 (MBD3 Produkte)

Synonyme: xmbd3 Protein, MGC69548 Protein, MBD3 Protein, DKFZp459N1635 Protein, AI181826 Protein, AU019209 Protein, methyl-CpG binding domain protein 3 S homeolog Protein, methyl-CpG binding domain protein 3 Protein, mbd3.S Protein, mbd3 Protein, MBD3 Protein, Mbd3 Protein

Hintergrund: Methyl-CpG-binding domain protein 3, also known as MBD3, is a member of the MBD family of transcriptional repressors. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Recombinant human MBD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 35.2 kDa (314aa) confirmed by MALDI-TOF

NCBI Accession: NP_003917

Pathways: Chromatin Binding