SIAH1 Protein (His tag)

Produktnummer ABIN1098362

Produktdetails

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SIAH1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: E3 ubiquitin-protein ligase SIAH1, SIAH1A

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 40% glycerol,1 MM DTT

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Andere Bezeichnung: SIAH1 (SIAH1 Produkte)

Synonyme: SIAH Protein, Siah1 Protein, Siah1a Protein, id:ibd2635 Protein, zgc:56026 Protein, SIAH1A Protein, siah Protein, E3 ubiquitin-protein ligase Siah1 Protein, E3 ubiquitin-protein ligase siah-1 Protein, siah E3 ubiquitin protein ligase 1 Protein, siah E3 ubiquitin protein ligase 1 L homeolog Protein, Tsp_03055 Protein, siah-1 Protein, Siah1 Protein, siah1 Protein, SIAH1 Protein, siah1.L Protein

Hintergrund: E3 ubiquitin-protein ligase SIAH1, also known as SIAH1, is a member of the seven in absentia homolog (SIAH) family. SIAH1 is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. SIAH1 contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. Recombinant human SIAH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 24.1 kDa (216aa), confirmed by MALDI-TOF

NCBI Accession: NP_003022