UBA5 Protein (His tag)

Produktnummer ABIN1098257

Produktdetails

Target: UBA5 (Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses UBA5 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: Ubiquitin-like modifier-activating enzyme 5, THIFP1, UBE1DC1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT, 10% glycerol, 50 mM NaCl

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: UBA5 (Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5))

Andere Bezeichnung: UBA5 (UBA5 Produkte)

Synonyme: cb972 Protein, ube1dc1 Protein, UBE1DC1 Protein, THIFP1 Protein, 5730525G14Rik Protein, AW240750 Protein, Ube1dc1 Protein, UBAL Protein, ubiquitin-like modifier activating enzyme 5 Protein, ubiquitin like modifier activating enzyme 5 Protein, ubiquitin-activating enzyme E1-like protein Protein, ubiquitin like modifier activating enzyme 5 S homeolog Protein, uba5 Protein, UBA5 Protein, LOC100270786 Protein, Uba5 Protein, uba5.S Protein

Hintergrund: UBA5 (ubiquitin-like modifier activating enzyme 5) belongs to the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are known to be covalently conjugated to a variety of cellular substrates via a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) plays a key role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 had been proved to activate an ubiquitin-like protein, ubiquitin-fold modifier 1 (Ufm1), by forming a high-energy thioester bond. It localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2. Recombinant human UBA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 47.4kDa (428aa) confirmed by MALDI-TOF

NCBI Accession: NP_079094