ALAD Protein (His tag)

Produktnummer ABIN7279740

Produktdetails

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses ALAD Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: Delta-aminolevulinic acid dehydratase, ALADH, PBGS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1 M NaCl

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Andere Bezeichnung: ALAD (ALAD Produkte)

Synonyme: DDBDRAFT_0190269 Protein, DDBDRAFT_0231415 Protein, DDB_0190269 Protein, DDB_0231415 Protein, ALAD Protein, ncf Protein, ALADH Protein, PBGS Protein, ALADR Protein, aminolevulinatedelta-dehydratase Protein, zgc:110219 Protein, Lv Protein, delta-aminolevulinate dehydratase Protein, aminolevulinate dehydratase Protein, delta-aminolevulinic acid dehydratase Protein, Delta-aminolevulinic acid dehydratase Protein, aminolevulinate dehydratase L homeolog Protein, aminolevulinate, delta-, dehydratase Protein, hemB Protein, ALAD Protein, PBGS Protein, STY0404 Protein, SAS1597 Protein, SACI_RS03725 Protein, PAAG_00299 Protein, VDBG_00315 Protein, HPPC_00830 Protein, MGYG_01952 Protein, hem2 Protein, alad.L Protein, Alad Protein, alad Protein

Hintergrund: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Recombinant human ALAD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 38.8 kDa (354aa), confirmed by MALDI-TOF

NCBI Accession: NP_000022