IFI30 Protein (His tag)
-
- Target Alle IFI30 Proteine anzeigen
- IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))
- Protein-Typ
- Recombinant
-
Spezies
- Human
-
Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses IFI30 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Aufreinigung
- purified by chromatography
- Reinheit
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product IFI30 Protein
-
-
- Kommentare
-
Synonyms: Gamma-interferon-inducible lysosomal thiol reductase, GILT, IFI-30, IP30
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Avoid repeated freezing and thawing cycles.
-
- Target
- IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))
- Andere Bezeichnung
- IFI30 (IFI30 Produkte)
- Synonyme
- GILT Protein, wu:fb12b04 Protein, zgc:101752 Protein, IP30 Protein, IFI-30 Protein, interferon, gamma-inducible protein 30 Protein, IFI30, lysosomal thiol reductase Protein, interferon gamma inducible protein 30 Protein, ifi30 Protein, IFI30 Protein, LOC100304719 Protein, Ifi30 Protein
- Hintergrund
- IFI30, also as known as gamma-interferon-inducible lysosomal thiol reductase, belongs to the GILT family. This protein is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. This protein facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. Recombinant human IFI30 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
- Molekulargewicht
- 22.5 kDa (199aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_006323
-