SEMA5A Protein (AA 23-765) (His tag)

Produktnummer ABIN1097496

Produktdetails

Target: SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 23-765

Spezies: Human

Quelle: Human Cells

Aufreinigungstag / Konjugat: Dieses SEMA5A Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Semaphorin-5A/SEMA5A (C-6His)

Sequenz: EAQGTTQCQR TEHPVISYKE IGPWLREFRA KNAVDFSQLT FDPGQKELVV GARNYLFRLQ LEDLSLIQAV EWECDEATKK ACYSKGKSKE ECQNYIRVLL VGGDRLFTCG TNAFTPVCTN RSLSNLTEIH DQISGMARCP YSPQHNSTAL LTAGGELYAA TAMDFPGRDP AIYRSLGILP PLRTAQYNSK WLNEPNFVSS YDIGNFTYFF FRENAVEHDC GKTVFSRAAR VCKNDIGGRF LLEDTWTTFM KARLNCSRPG EVPFYYNELQ STFFLPELDL IYGIFTTNVN SIAASAVCVF NLSAIAQAFS GPFKYQENSR SAWLPYPNPN PHFQCGTVDQ GLYVNLTERN LQDAQKFILM HEVVQPVTTV PSFMEDNSRF SHVAVDVVQG REALVHIIYL ATDYGTIKKV RVPLNQTSSS CLLEEIELFP ERRREPIRSL QILHSQSVLF VGLREHVVKI PLKRCQFYRT RSTCIGAQDP YCGWDVVMKK CTSLEESLSM TQWEQSISAC PTRNLTVDGH FGVWSPWTPC THTDGSAVGS CLCRTRSCDS PAPQCGGWQC EGPGMEIANC SRNGGWTPWT SWSPCSTTCG IGFQVRQRSC SNPTPRHGGR VCVGQNREER YCNEHLLCPP HMFWTGWGPW ERCTAQCGGG IQARRRICEN GPDCAGCNVE YQSCNTNPCP ELKKTTPWTP WTPVNISDNG GHYEQRFRYT CKARLADPNL LEVGRQRIEM RYCSSDGTSG CSTLDHHHHH H

Produktmerkmale: Recombinant Human Semaphorin-5A/SEMA5A produced by transfected human cells is a secreted protein with sequence (Glu23-Thr765) of Human SEMA5A fused with a polyhistidine tag at the C-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 0.1 mM EDTA, 0.05 % Tween 20, pH 7.2.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 months

Antigendetails

Target: SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))

Andere Bezeichnung: semaphorin-5a (SEMA5A Produkte)

Synonyme: wu:fa95e03 Protein, wu:fi41a04 Protein, SEMAF Protein, semF Protein, 5930434A13 Protein, 9130201M22Rik Protein, AI464145 Protein, Semaf Protein, semaphorin 5A Protein, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A Protein, semaphorin-5A Protein, SEMA5A Protein, sema5a Protein, Sema5a Protein, LOC100516117 Protein

Sub Type: Fusionprotein

Hintergrund: Semaphorin-5A (SEMA5A) is a member of the Semaphorin family of axon guidance molecules. SEMA5A is a 140 kDa protein. Class 5 Semaphorins are type I transmembrane glycoproteins with an N- terminal Sema domain and multiple juxtamembrane type 1 Thrombospondin (TSP) repeats within their extracellular domains. SEMA5A is expressed in neuroepithelial cells surrounding retinal axons, oligodendrocytes, the base of limb buds, the mesoderm surrounding cranial vessels , and the cardiac atrial septum and endocardial cushions, Human SEMA5A cDNA encodes a signal sequence, a extracellular domain (ECD), a transmembrane sequence and an cytoplasmic portion. SEMA5A mutations have been implicated in the genetic syndrome,cri-du-chat,while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson. The expression of SEMA5A may be upregulated in metastatic cancer cells and downregulated in autism.
Alternative Names: Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF

Molekulargewicht: 84.71 kDa

UniProt: Q13591