Protein phosphatase 1 (AA 1-171), (Regulatory Subunit 1A) protein (His tag)

Produktnummer ABIN1097388

Produktdetails

Target: Protein phosphatase 1

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-171, Regulatory Subunit 1A

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Recombinant Human Protein Phosphatase 1 Regulatory Subunit 1A/PPP1R1A (C-6His)

Sequenz: MEQDNSPQKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEI DEDRIPNPHL KSTLAMSPRQ RKKMTRITPT MKELQMMVEH HLGQQQQGEE PEGAAESTGT QESRPPGIPD TEVESRLGTS GTAKKTAECI PKTHERGSKE PSTKEPSTHI PPLDSKGANS VLEHHHHHH

Produktmerkmale: Recombinant Human Protein Phosphatase 1 Regulatory Subunit 1A/PPP1R1A is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val171) of Human PPP1R1A.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 1 mM DTT, 50 % Glycerol, pH 8.5.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: Protein phosphatase 1

Synonyme: Ppp1cb Protein, protein phosphatase 1 catalytic subunit beta Protein, PPP1CB Protein

Hintergrund: Protein Phosphatase 1 Regulatory Subunit 1A (PPP1R1A) is an inhibitor of protein-phosphatase 1. PPP1R1A is a cellular regulator of eIF2 alpha phosphorylation. In hormonal control of glycogen metabolism, IPP-1 protein plays important function. Hormones can elevate intracellular cAMP level and elevate IPP-1 activity. PPP1R1A activation caused cAMP increase , cAMP control over proteins that are not directly phosphorylated by PKA following a rise in intracellular calcium. IPP-1 is inactivated by calcineurin (PP2B). Multiple domains in IPP-1 target cellular PP1 complexes.
Alternative Names: Protein Phosphatase 1 Regulatory Subunit 1A, Protein Phosphatase Inhibitor 1, I-1, IPP-1, PPP1R1A, IPP1

Molekulargewicht: 19.98 kDa

UniProt: Q13522