Recombinant Human Cytotoxic and Regulatory T-Cell Molecule/CRTAM is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ser18-Ser286) of Human CRTAM fused with a polyhistidine tag at the C-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
Crystallography grade
CRTAM
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
CRTAM
Spezies: Cynomolgus
Wirt: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
WB
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Haltbarkeit
3 months
Target
CRTAM
(Cytotoxic and Regulatory T Cell Molecule (CRTAM))
TAD Protein, cTADS Protein, MGC82176 Protein, CRTAM Protein, CD355 Protein, cytotoxic and regulatory T-cell molecule Protein, cytotoxic and regulatory T-cell molecule L homeolog Protein, cytotoxic and regulatory T cell molecule Protein, CRTAM Protein, crtam.L Protein, Crtam Protein
Sub Type
Fusionprotein
Hintergrund
Cytotoxic and Regulatory T-Cell Molecule (CRTAM) is a member of Nectin family under the immunoglobulin superfamily that is expressed by activated CD8+ and NK T cells. CRTAM is found in spleen, thymus, small intestine, peripheral blood, and it is highly expressed by Purkinje cells of the cerebellum. CRTAM is a type I transmembrane glycoprotein containing one Ig-like C2-type domain and one Ig-like V-type domain in its extracellular domain, while its cytoplasmic region shows a potential class I PDZ domain. CRTAM is expressed as a homodimer on the cell surface but does not show homotypic binding in trans. The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions. IGSF4 and T cell receptor coengagement of CD8+ cells expressiong CRTAM induces increased IFNgamma or IL-22 production. Alternative Names: Cytotoxic and Regulatory T-Cell Molecule, Class-I MHC-Restricted T-Cell-Associated Molecule, CD355, CRTAM