Recombinant Human SLAM Family Member 7/SLAMF7 produced by transfected human cells is a secreted protein with sequence (Ser23-Ser225) of Human SLAMF7 fused with a polyhistidine tag at the C-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
SLAMF7
Spezies: Rhesusaffen
Wirt: Human Cells
Recombinant
>95 % as determined by reducing SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
RGD1560190 Protein, SLAMF7 Protein, slaf7 Protein, 19A Protein, CD319 Protein, CRACC Protein, CS1 Protein, 19A24 Protein, 4930560D03Rik Protein, SLAM family member 7 Protein, Slamf7 Protein, SLAMF7 Protein, slaf7 Protein
Sub Type
Fusionprotein
Hintergrund
SLAMF7 is a single-pass type I membrane protein and contains 1 Ig-like C2-type (immunoglobulin-like) domain. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-cell lines, or T-cell lines. Although the cytoplasmic domain of CS1 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM)-associated protein (SAP/SH2D1A), it activates NK cells in the absence of a functional SAP. SLAMF7 positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. However, in the absence of EAT-2, CRACC potently inhibited natural killer cell function. It was also inhibitory in T cells, which are typically devoid of EAT-2. Thus, SLAMF7 can exert activating or inhibitory influences on cells of the immune system depending on cellular context and the availability of effector proteins. Alternative Names: SLAM Family Member 7, CD2 Subset 1, CD2-Like Receptor-Activating Cytotoxic Cells, CRACC, Membrane Protein FOAP-12, Novel Ly9, Protein 19A, CD319, SLAMF7, CS1