ASS1 Protein (AA 1-412) (His tag)

Produktnummer ABIN1096047

Produktdetails

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-412

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses ASS1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Argininosuccinate Synthase/ASS1 (N-6His)

Sequenz: MGSSHHHHHH SSGLVPRGSH MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF IEDVSREFVE EFIWPAIQSS ALYEDRYLLG TSLARPCIAR KQVEIAQREG AKYVSHGATG KGNDQVRFEL SCYSLAPQIK VIAPWRMPEF YNRFKGRNDL MEYAKQHGIP IPVTPKNPWS MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNTP DILEIEFKKG VPVKVTNVKD GTTHQTSLEL FMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIAK SQERVEGKVQ VSVLKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPTDATG FININSLRLK EYHRLQSKVT AK

Produktmerkmale: Recombinant Human Argininosuccinate Synthase/ASS1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Lys412) of Human ASS1 fused with a 6His tag at the C-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 50 mM Imidazole, 1 mM DTT, 40 % Glycerol, pH 7.5.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Andere Bezeichnung: Argininosuccinate Synthase/ASS1 (ASS1 Produkte)

Synonyme: ASS Protein, CTLN1 Protein, AA408052 Protein, Ass-1 Protein, fold Protein, ASSA Protein, Ass Protein, ass Protein, zgc:92051 Protein, wu:fb95a04 Protein, wu:fc01e08 Protein, argininosuccinate synthase 1 Protein, argininosuccinate synthetase 1 Protein, argininosuccinate synthase 1 S homeolog Protein, ASS1 Protein, Ass1 Protein, ass1.S Protein, ass1 Protein

Sub Type: Fusionprotein

Hintergrund: Argininosuccinate Synthase (ASS1) is an urea cycle enzyme with a tetrameric structure composed of identical subunits. ASS1 is involved in the synthesis of arginine and catalyzes that condensation of citrulline and aspartate to argininosuccinate using ATP. ASS1 is important to the urea cycle as it catalyzes the important second last step in the arginine biosynthetic pathway. ASS1 mainly expressed in periportal hepatocytes, but also in most other body tissues. A deficiency of ASS1 causes citrullinemia (CTLN1), an autosomal recessive disease which is characterized by severe vomiting spells and mental retardation.
Alternative Names: Argininosuccinate Synthase, Citrulline--Aspartate Ligase, ASS1, ASS

Molekulargewicht: 42.8 kDa

UniProt: P00966

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin