Recombinant Human Eukaryotic Translation Initiation Factor 4E-Binding Protein 2/EIF4EBP2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ile120) of Human EIF4EBP2 fused with a 6His tag at the N-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
EIF4EBP2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0 .
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Haltbarkeit
3 months
Target
eIF4EBP2 (EIF4EBP2)
(Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2))
4ebp2 Protein, MGC75579 Protein, wu:fa55e11 Protein, wu:fp45e09 Protein, MGC78987 Protein, EIF4EBP2 Protein, eIF-4E Protein, Tb06.28P18.1010 Protein, 4EBP2 Protein, PHASII Protein, 2810011I19Rik Protein, 4E-BP2 Protein, AA792569 Protein, BC010348 Protein, PHAS-II Protein, eukaryotic translation initiation factor 4E binding protein 2 Protein, eukaryotic translation initiation factor 4E binding protein 2 L homeolog Protein, eukaryotic initiation factor 4E Protein, eukaryotic translation initiation factor 4E Protein, putative eukaryotic translation initiation factor 4e Protein, eif4ebp2 Protein, Eif4ebp2 Protein, eif4ebp2.L Protein, EIF4EBP2 Protein, eIF-4E Protein, Tb927.6.1870 Protein, LMJF_30_0450 Protein
Sub Type
Fusionprotein
Hintergrund
Eukaryotic Translation Initiation Factor 4E-Binding Protein 2 (EIF4EBP2) is a member of the Eukaryotic Translation Initiation Factor 4E Binding Protein Family. EIF4EBP2 regulates eIF4E activity by preventing its assembly into the eIF4F complex, mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway. This regulation of is associated to cell proliferation, cell differentiation and viral infection. Phosphorylated EIF4EBP2 on serine and threonine residues in response to insulin, EGF and PDGF. Alternative Names: Eukaryotic Translation Initiation Factor 4E-Binding Protein 2, 4E-BP2, eIF4E-Binding Protein 2, EIF4EBP2