Seryl-tRNA Synthetase (SARS) (AA 2-233), (partial) protein (GST tag)

Produktnummer ABIN1047631

Produktdetails

Target: Seryl-tRNA Synthetase (SARS)

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-233, partial

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: GST tag

Applikation: ELISA

Sequenz: VLDLDLFRVD KGGDPALIRE TQEKRFKDPG LVDQLVKADS EWRRCRFRAD NLNKLKNLCS KTIGEKMKKK EPVGDDESVP ENVLSFDDLT ADALANLKVS QIKKVRLLID EAILKCDAER IKLEAERFEN LREIGNLLHP SVPISNDEDV DNKVERIWGD CTVRKKYSHV DLVVMVDGFE GEKGAVVAGS RGYFLKGVLV FLEQALIQYA LRTLGSRGYI PIYTPFFMRK EV

Produktmerkmale: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Reinheit: 90 %

Anwendungsinformationen

Kommentare:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handhabung: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Referenzen

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, (2003) (PubMed).

Vincent, Tarbouriech, Härtlein: "Genomic organization, cDNA sequence, bacterial expression, and purification of human seryl-tRNA synthase." in: European journal of biochemistry / FEBS, Vol. 250, Issue 1, pp. 77-84, (1998) (PubMed).

Antigendetails

Target: Seryl-tRNA Synthetase (SARS)

Andere Bezeichnung: Seryl-tRNA synthetase,cytoplasmic protein (SARS Produkte)

Synonyme: SERRS Protein, SERS Protein, Sars1 Protein, Strs Protein, serRS Protein, SerRS Protein, wu:fk51f08 Protein, zgc:92152 Protein, seryl-tRNA synthetase Protein, seryl-aminoacyl-tRNA synthetase Protein, SARS Protein, Sars Protein, sars Protein

Hintergrund: Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).

Molekulargewicht: 53.8 kD

UniProt: P49591