MYL6 Protein (AA 3-151, partial) (GST tag)

Produktnummer ABIN1047437

Produktdetails

Target: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Protein-Typ: Recombinant

Proteineigenschaft: AA 3-151, partial

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses MYL6 Protein ist gelabelt mit GST tag.

Applikation: ELISA

Sequenz: DFTEDQTAEF KEAFQLFDRT GDGKILYSQC GDVMRALGQN PTNAEVLKVL GNPKSDEMNV KVLDFEHFLP MLQTVAKNKD QGTYEDYVEG LRVFDKEGNG TVMGAEIRHV LVTLGEKMTE EEVEMLVAGH EDSNGCINYE AFVRHILSG

Produktmerkmale: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Reinheit: 90 %

Anwendungsinformationen

Kommentare:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handhabung: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Referenzen

Watanabe, Kohri, Takaishi, Horie, Higashihara: "Molecular cloning and sequencing of myosin light chains in human megakaryoblastic leukemia cells." in: Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi, Vol. 37, Issue 1, pp. 25-38, (2001) (PubMed).

Bora, Bora, Wu, Kaplan, Lange: "Molecular cloning, sequencing, and characterization of smooth muscle myosin alkali light chain from human eye cDNA: homology with myocardial fatty acid ethyl ester synthase-III cDNA." in: Genomics, Vol. 19, Issue 1, pp. 186-8, (1994) (PubMed).

Hailstones, Gunning: "Characterization of human myosin light chains 1sa and 3nm: implications for isoform evolution and function." in: Molecular and cellular biology, Vol. 10, Issue 3, pp. 1095-104, (1990) (PubMed).

Lenz, Lohse, Seidel, Arnold: "The alkali light chains of human smooth and nonmuscle myosins are encoded by a single gene. Tissue-specific expression by alternative splicing pathways." in: The Journal of biological chemistry, Vol. 264, Issue 15, pp. 9009-15, (1989) (PubMed).

Antigendetails

Target: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Andere Bezeichnung: Myosin light polypeptide 6 protein (MYL6 Produkte)

Synonyme: ESMLC Protein, LC17 Protein, LC17-GI Protein, LC17-NM Protein, LC17A Protein, LC17B Protein, MLC-3 Protein, MLC1SM Protein, MLC3NM Protein, MLC3SM Protein, zgc:103624 Protein, MLC3nm Protein, Myln Protein, myosin light chain 6 Protein, myosin, light chain 6, alkali, smooth muscle and non-muscle Protein, myosin light chain 6 L homeolog Protein, myosin, light polypeptide 6, alkali, smooth muscle and non-muscle Protein, MYL6 Protein, Myl6 Protein, myl6 Protein, myl6.L Protein

Hintergrund: Regulatory light chain of myosin. Does not bind calcium.

Molekulargewicht: 44.1 kD

UniProt: P60660