When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
ABCC5
(ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 5 (ABCC5))
Synonyme
ABC33 Peptide, EST277145 Peptide, MOAT-C Peptide, MOATC Peptide, MRP5 Peptide, SMRP Peptide, pABC11 Peptide, 2900011L11Rik Peptide, AI132311 Peptide, Abcc5a Peptide, Abcc5b Peptide, Mrp5 Peptide, abc33 Peptide, abcc5 Peptide, moat-c Peptide, moatc Peptide, mrp5 Peptide, pabc11 Peptide, smrp Peptide, ATP binding cassette subfamily C member 5 Peptide, ATP-binding cassette, sub-family C (CFTR/MRP), member 5 Peptide, ATP binding cassette subfamily C member 5 L homeolog Peptide, ABCC5 Peptide, Abcc5 Peptide, abcc5.L Peptide