When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
NR2F6
(Nuclear Receptor Subfamily 2, Group F, Member 6 (NR2F6))
Synonyme
EAR-2 Peptide, EAR2 Peptide, ERBAL2 Peptide, AV090102 Peptide, COUP-TF3 Peptide, Erbal2 Peptide, COUP(II) Peptide, NR2F6 Peptide, couptf2 Peptide, fc94g11 Peptide, wu:fc94g11 Peptide, zgc:77259 Peptide, ear-2 Peptide, ear2 Peptide, erbal2 Peptide, nr2f6l Peptide, wu:fc72d04 Peptide, zgc:77260 Peptide, nuclear receptor subfamily 2 group F member 6 Peptide, nuclear receptor subfamily 2, group F, member 6 Peptide, nuclear receptor subfamily 2, group F, member 6a Peptide, nuclear receptor subfamily 2 group F member 6 L homeolog Peptide, nuclear receptor subfamily 2, group F, member 6b Peptide, NR2F6 Peptide, Nr2f6 Peptide, nr2f6a Peptide, nr2f6.L Peptide, nr2f6 Peptide, nr2f6b Peptide