When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
SLC7A5
(Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 5 (SLC7A5))
Synonyme
4F2LC Peptide, CD98 Peptide, D16S469E Peptide, E16 Peptide, LAT1 Peptide, MPE16 Peptide, hLAT1 Peptide, D0H16S474E Peptide, TA1 Peptide, JHI-21 Peptide, SLC7A5 Peptide, LAT-1 Peptide, slc7a5a Peptide, 4F2 Peptide, 4F2HC Peptide, AI314110 Peptide, Cd98 Peptide, Ly-10 Peptide, Ly-m10 Peptide, Ly10 Peptide, Mdu1 Peptide, Mgp-2hc Peptide, NACAE Peptide, solute carrier family 7 member 5 Peptide, solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 Peptide, solute carrier family 7 (amino acid transporter light chain, L system), member 5 Peptide, solute carrier family 7 member 5 S homeolog Peptide, solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 Peptide, SLC7A5 Peptide, Slc7a5 Peptide, slc7a5.S Peptide, Slc3a2 Peptide