DYKDDDDK Tag peptide (DYKDDDDK Tag)

Details zu Produkt Nr. ABIN1607595, Anbieter: Anmelden zum Anzeigen
Proteinname
Spezies
Tag
1
Aufreinigungstag / Konjugat
DYKDDDDK Tag
Applikation
Control Peptide (CP), Immunoprecipitation (IP), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Reinheit > 90 % specific peptide by HPLC and MS.
Endotoxin-Niveau Low Endotoxin : No
Proteinname
Substanzklasse Tag
Hintergrund The DYKDDDDK peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free DYKDDDDK Peptide. Synonyms: synthetic DYKDDDDK peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide
Applikations-hinweise A working concentration of 500 μg/mL for triple DYKDDDDK peptide is typically used for competitive elution of DYKDDDDK containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK DYKDDDDK affinity gel PROTOCOL for specific instructions for use.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution 0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4
Konzentration 1 mg/mL
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 2 - 8 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Haltbarkeit Expiration date is six (6) months from date of opening.
Bilder des Herstellers
Western Blotting (WB) image for DYKDDDDK Tag peptide (DYKDDDDK Tag) (ABIN1607595) SDS-PAGE of Anti-DYKDDDDK Affinity Gel. Lane 1: Cell lysate before purification. Lan...
Western Blotting (WB) image for DYKDDDDK Tag peptide (DYKDDDDK Tag) (ABIN1607595) SDS-PAGE of Anti-DYKDDDDK Affinity Gel. Lane 1: crude lysate containing over-express...
SDS-PAGE (SDS) image for DYKDDDDK Tag peptide (DYKDDDDK Tag) (ABIN1607595) SDS-PAGE of Anti-DYKDDDDK affinity gel using DYKDDDDK control peptide. Lane 1: DYKDDD...