Human Tryptase/TPSAB1,B2 EZ Set™ ELISA Kit (DIY Antibody Pairs)
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- Target Alle TPSB2 Produkte
- TPSB2 (Tryptase beta 2 (TPSB2))
- Bindungsspezifität
- AA 1-275
- Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Wirt
- Kaninchen
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects Tryptase/TPSAB1,B2 in Human samples.
- Marke
- EZ-Set™
- Spezifität
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Expression system for standard: NSO
Immunogen sequence: M1-V275 - Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Produktmerkmale
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Tissue Specificity: TPSAB1: Isoform 1 and isoform 2 are expressed in lung,stomach, spleen, heart and skin, in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level).
- Bestandteile
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- Benötigtes Material
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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- Kommentare
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Haltbarkeit
- 12 months
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- Target
- TPSB2 (Tryptase beta 2 (TPSB2))
- Andere Bezeichnung
- TPSB2 (TPSB2 Produkte)
- Synonyme
- TPS2 Matched Antibody Pair, tryptaseB Matched Antibody Pair, tryptaseC Matched Antibody Pair, Mcpt6 Matched Antibody Pair, tryptase beta 2 (gene/pseudogene) Matched Antibody Pair, tryptase beta 2 Matched Antibody Pair, TPSB2 Matched Antibody Pair, Tpsb2 Matched Antibody Pair
- Hintergrund
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Protein Function: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. .
Background: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles, TPSB2, which represents the beta-II and beta-III tryptase alleles, TPSG1, and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.
Synonyms: Tryptase beta-2,Tryptase-2,3.4.21.59,Tryptase II,TPSB2,TPS2,
Full Gene Name: Tryptase beta-2
Cellular Localisation: Secreted. Released from the secretory granules upon mast cell activation. - UniProt
- P20231
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