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|Antigen||Nuclear Factor-KB P65 (NFkBP65) Antikörper|
|Konjugat||Dieser p65 Antikörper ist unkonjugiert Alternativen|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|Hersteller||Anmelden zum Anzeigen|
Produktdetails anti-p65 AntikörperTarget Details p65 Anwendungsinformationen Handhabung Bilder
|Spezifität||This phospho-specific polyclonal antibody is specific for phosphorylated pSer529 Human p65. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pSer529 phosphorylated p65 from Mouse, Rat or other species has not been determined. Anti-phospho NFkB antibody reacts human pS529 p65 and shows minimal reactivity by Western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.|
|Produktmerkmale||Concentration: 75.0 mg/mL (by Refractometry)|
|Reinigung||Delipidation and Defibrination|
|Immunogen||NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Sequence information: PNGLLpSGDEDFC|
Target Details p65Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
|Andere Bezeichnung||RELA / NF-kB p65 (NFkBP65 Antibody Abstract)|
|Hintergrund||NFkB (Nuclear Factor NF-kappa-B) is a pleiotropic transcription factor that plays a role in many biological processes, including inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. It is found as a homo- or heterodimeric complex containing the Rel-like domain containing proteins NFkB p65 (RELA/p65), RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The heterodimeric NFkB p65/p50 complex is the most abundant one. The dimers bind to kappa-B sites at their target genes, with the affinity of the interaction dependent on the subunit composition of the dimer. Furthermore, different dimers act as transcriptional activators or repressors, with the NFkB p65/p50 and p65-c-Rel complexes acting as activators. NFkB activity is controlled by several different mechanisms, including post-translational modifications, subcellular localisation and interactions with other coactivators or corepressors. NFkB complexes are held in the cytoplasm in an inactive state by interaction with members of the NFkB inhibitor (IkB) family. Typically, phosphorylation of IkB by IkB kinases (IKKs) in response to different activators leads to degradation of the inhibitor, allowing NFkB to translocate into the nucleus. The inhibitory effect of IkBs is primarily exerted through their interaction with NfKB p65. NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65|
|Pathways||NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades|
AnwendungsinformationenProduktdetails anti-p65 Antikörper Target Details p65 Handhabung Bilder zurück nach oben
For immunoblotting a 1: 1,000 dilution is recommended. A 65 kDa band corresponding to human p65 is detected. HeLa cells or TNF inducible KBM-5cells can be used as a positive control. For ELISA a 1: 5,000 to 1: 10,000 dilution is recommended. Researchers should determineoptimal titers for other applications. Although not tested, this antibody is likely functional in immunohistochemistry andimmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Beschränkungen||Nur für Forschungszwecke einsetzbar|
HandhabungProduktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Bilder zurück nach oben
|Buffer||0.09 % (w/v) Sodium Azide|
|Vorsichtsmaßnahmen||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handhabung||Avoid repeated freezing and thawing.|
|Lagerung||4 °C/-20 °C|
|Informationen zur Lagerung||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|