anti-Human ERK2 Antikörper für Enzyme Immunoassay

Recommended ERK2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antikörper
  • ERK
  • ERK2
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • zERK2
  • MAPK1
  • Xp42
  • erk
  • erk2
  • ert1
  • mapk
  • mapk1b
  • mapk2
  • mpk1
  • prkm1
  • prkm2
  • mapk1
  • mapk1a
  • xp42
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 1
  • mitogen activated protein kinase 1
  • mitogen-activated protein kinase 1 L homeolog
  • mitogen-activated protein kinase 1 S homeolog
  • MAPK1
  • Mapk1
  • mapk1
  • mapk1.L
  • mapk1.S
  • PfMAP1
  • MPK1
  • PVX_084965
  • LOC9328617
  • Tsp_01601
Epitop
C-Term
58
21
16
11
9
8
8
8
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human
352
150
119
51
31
19
14
11
10
9
9
7
5
5
2
2
2
2
2
1
1
Wirt
Kaninchen
169
167
16
8
3
Klonalität
Polyklonal
Konjugat
Dieser ERK2 Antikörper ist unkonjugiert
10
9
8
7
7
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Applikation
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
329
161
96
83
82
60
53
27
23
9
5
3
3
2
2
1
1
1
1
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN400970
$ 533.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN492382 EIA IF WB Mouse IgG2b AA 1-360, N-Term Anmelden zum Anzeigen AT1A4 0
1 ABIN126833 EIA WB Mouse IgG1 N-Term Anmelden zum Anzeigen 6H3 1
1 ABIN126832 EIA IP WB Mouse IgG1 C-Term Anmelden zum Anzeigen 6G11 0
1 ABIN5552223 EIA IHC (p) WB Rabbit Ig Fraction C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN5552208 EIA IHC (p) WB Rabbit Ig Fraction Middle Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN400733 EIA IHC (fro) IHC (p) WB Mouse IgG2a Anmelden zum Anzeigen 4C11C11C4 0
1 ABIN492351 EIA IP WB Biotin Mouse IgG1 C-Term Anmelden zum Anzeigen 6G11 0
1 ABIN492352 EIA WB Biotin Mouse IgG1 N-Term Anmelden zum Anzeigen 6H3 0
1 ABIN641241 CA DB EIA IA ISt ELISA WB Rabbit Anmelden zum Anzeigen Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antikörper
Epitop C-Term
(58), (21), (16), (11), (9), (8), (8), (8), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(352), (150), (119), (51), (31), (19), (14), (11), (10), (9), (9), (7), (5), (5), (2), (2), (2), (2), (2), (1), (1)
Wirt Kaninchen
(169), (167), (16), (8), (3)
Klonalität
Polyklonal
Konjugat Dieser ERK2 Antikörper ist unkonjugiert
(10), (9), (8), (7), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Applikation Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(329), (161), (96), (83), (82), (60), (53), (27), (23), (9), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-ERK2 Antikörper

Target Details ERK2 Anwendungsinformationen Handhabung Referenzen für anti-ERK2 Antikörper (ABIN400970) Bilder
Spezifität This antibody reacts to p42 MAP Kinase (ERK2) protein. No reactivity is observed against p44 MAP Kinase (ERK1). Cross reactivity is expected with p44 MAP Kinase (ERK1) proteins from human and mouse sources.
Kreuzreaktivität (Details) Species reactivity (expected):Rat.
Species reactivity (tested):Human.
Reinigung Serum
Immunogen This whole rabbit antiserum was prepared by repeated immunizations with a GST fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
Isotyp IgG

Target Details ERK2

Produktdetails anti-ERK2 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-ERK2 Antikörper (ABIN400970) Bilder zurück nach oben
Antigen
Andere Bezeichnung ERK2 (MAPK1 Antibody Abstract)
Hintergrund Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK's) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK's form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the upregulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2. In vertebrates the mitogeninduced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
Gen-ID 5594
NCBI Accession NP_002736
UniProt P28482
Pathways MAPK Signalweg, RTK Signalweg, Apoptose, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagie, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Anwendungsinformationen

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Handhabung Referenzen für anti-ERK2 Antikörper (ABIN400970) Bilder zurück nach oben
Applikationshinweise ELISA: 1/2000 to 1/10000. Western Blot: 1/1000-1/2000. Immunopreicpitation: 5.0 μg/mg sample.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Anwendungsinformationen Referenzen für anti-ERK2 Antikörper (ABIN400970) Bilder zurück nach oben
Format Liquid
Konzentration 85 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Referenzen für anti-ERK2 Antikörper (ABIN400970)

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Asthagiri, Reinhart, Horwitz, Lauffenburger: "The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis." in: Journal of cell science, Vol. 113 Pt 24, pp. 4499-510, 2001

Hoffmann, Baillie, MacKenzie, Yarwood, Houslay: "The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579." in: The EMBO journal, Vol. 18, Issue 4, pp. 893-903, 1999

Garrington, Johnson: "Organization and regulation of mitogen-activated protein kinase signaling pathways." in: Current opinion in cell biology, Vol. 11, Issue 2, pp. 211-8, 1999

Elion: "Routing MAP kinase cascades." in: Science (New York, N.Y.), Vol. 281, Issue 5383, pp. 1625-6, 1998

Pelech, Charest: "MAP kinase-dependent pathways in cell cycle control." in: Progress in cell cycle research, Vol. 1, pp. 33-52, 1998

Bilder

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Anwendungsinformationen Handhabung Referenzen für anti-ERK2 Antikörper (ABIN400970) zurück nach oben
Bilder des Herstellers
 image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (C-Term) antibody (ABIN400970) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (C-Term) antibody