anti-Human C1QA Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended C1QA Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Complement Component 1, Q Subcomponent, A Chain (C1QA) Antikörper
  • zgc:109903
  • C1QA
  • AI255395
  • C1q
  • complement C1q A chain
  • complement component 1, q subcomponent, A chain
  • complement component 1, q subcomponent, alpha polypeptide
  • C1QA
  • c1qa
  • C1qa
Epitop
C-Term
8
8
7
6
4
4
3
3
3
2
2
1
1
Reaktivität
Human
68
18
10
2
2
1
1
1
Wirt
Kaninchen
52
20
5
3
1
Klonalität
Monoklonal
Konjugat
Dieser C1QA Antikörper ist unkonjugiert
4
3
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
67
28
27
12
9
6
6
6
4
3
2
1
1
1
Optionen
Hersteller
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Produktnummer ABIN1019924
$ 1,900.03
Zzgl. Versandkosten $45.00
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General

Antigen Complement Component 1, Q Subcomponent, A Chain (C1QA) Antikörper
Epitop C-Term
(8), (8), (7), (6), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Reaktivität Human
(68), (18), (10), (2), (2), (1), (1), (1)
Wirt Kaninchen
(52), (20), (5), (3), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser C1QA Antikörper ist unkonjugiert
(4), (3), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(67), (28), (27), (12), (9), (6), (6), (6), (4), (3), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-C1QA Antikörper

Target Details C1QA Anwendungsinformationen Handhabung Bilder
Verwendungszweck This antibody is designed for the specific localization of human C1qA using IHC techniques in formalin-fixed, paraffin- embedded tissue sections.
Produktmerkmale This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
Reinigung Ready-to-use, Prediluted, obtained from culture supernatant
Benötigtes Material All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope.
Immunogen A synthetic peptide corresponding to residues near the C terminus of human C1q (subunit A) was used as an immunogen.
Klon EPR2980Y
Isotyp IgG
Plasmids, Primers & others

Target Details C1QA

Produktdetails anti-C1QA Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung C1qA (C1QA Antibody Abstract)
Pathways Komplementsystem

Anwendungsinformationen

Produktdetails anti-C1QA Antikörper Target Details C1QA Handhabung Bilder zurück nach oben
Kommentare

Staining pattern: Secreted. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from kidney or tonsil.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for C1qA.

Protokoll Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples.
Testdurchführung Antigen retrieval: HIER Citrate buffer pH 6.5
Working dilution: (only for concentrates) 1:25 – 1:200
Incubation: 30 min, RT
Control Tissue: Kidney, tonsil
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-C1QA Antikörper Target Details C1QA Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Buffer The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.