We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Complement Component 1, Q Subcomponent, A Chain (C1QA) Antikörper|
|Konjugat||Dieser C1QA Antikörper ist unkonjugiert Alternativen|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|Hersteller||Anmelden zum Anzeigen|
Produktdetails anti-C1QA AntikörperTarget Details C1QA Anwendungsinformationen Handhabung Bilder
|Verwendungszweck||This antibody is designed for the specific localization of human C1qA using IHC techniques in formalin-fixed, paraffin- embedded tissue sections.|
|Produktmerkmale||This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.|
|Reinigung||Ready-to-use, Prediluted, obtained from culture supernatant|
|Benötigtes Material||All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope.|
|Immunogen||A synthetic peptide corresponding to residues near the C terminus of human C1q (subunit A) was used as an immunogen.|
|Plasmids, Primers & others|
Target Details C1QAProduktdetails anti-C1QA Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
|Andere Bezeichnung||C1qA (C1QA Antibody Abstract)|
AnwendungsinformationenProduktdetails anti-C1QA Antikörper Target Details C1QA Handhabung Bilder zurück nach oben
Staining pattern: Secreted. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.
Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples.
Antigen retrieval: HIER Citrate buffer pH 6.5
Working dilution: (only for concentrates) 1:25 – 1:200
Incubation: 30 min, RT
Control Tissue: Kidney, tonsil
|Beschränkungen||Nur für Forschungszwecke einsetzbar|
HandhabungProduktdetails anti-C1QA Antikörper Target Details C1QA Anwendungsinformationen Bilder zurück nach oben
|Buffer||The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)|
|Vorsichtsmaßnahmen||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Informationen zur Lagerung||Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.|