IL-6 ELISA Kit

Produktnummer ABIN8057094

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus IL-6.

Kurzübersicht für IL-6 ELISA Kit (ABIN8057094)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.43 pg/mL - 100 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Güteklasse: High Sensitivity

Produktdetails IL-6 ELISA Kit

Untere Nachweisgrenze: 0.43 pg/mL

Analytische Methode: Quantitative

Spezifität: IL-6

Bestandteile:

• Plate
• Standard
• Diluent

Benötigtes Material: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-6.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Details zu IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Andere Bezeichnung: IL-6

Hintergrund: Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Mature mouse IL-6 is 187 amino acids (aa) in length and shares 39 % and 85 % aa sequence identity with human and rat IL-6. IL-6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacteriumStreptococcus pneumoniae. IL-6 stimulates the inflammatory and auto-immune processes in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Behcet's disease, and rheumatoid arthritis. Advanced/ metastatic cancer patients have higher levels of IL-6 in their blood.

Gen-ID: 16193

NCBI Accession: NM_031168

UniProt: P08505

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome